Nanoparticle displacement assay with electrochemical nanopore-based sensors

Gergely Lautner, Mónika Plesz, Gyula Jágerszki, P. Fürjes, R. Gyurcsányi

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

The proof of concept of a nanoparticle displacement assay that enables the use of large diameter nanopores for the detection of targets of smaller molecular dimensions is presented. We hypothesized that an inherent signal amplification should arise from the selective displacement of nanoparticles preloaded in a nanopore by a much smaller molecular target. The method is demonstrated using peptide nucleic acid (PNA)-functionalized gold nanopore arrays in which short DNA-modified gold nanoparticles are anchored by weak interaction. Complementary microRNAs are detected via the resistance change caused by competitive displacement of nanoparticles from the PNA-functionalized nanopores.

Original languageEnglish
Pages (from-to)13-17
Number of pages5
JournalElectrochemistry Communications
Volume71
DOIs
Publication statusPublished - Oct 1 2016

Fingerprint

Nanopores
Assays
Peptide Nucleic Acids
Nanoparticles
Sensors
Gold
MicroRNAs
Amplification
DNA

Keywords

  • Displacement assay
  • microRNA
  • Nanoparticle
  • NASBA
  • PNA
  • Solid state nanopore

ASJC Scopus subject areas

  • Electrochemistry

Cite this

Nanoparticle displacement assay with electrochemical nanopore-based sensors. / Lautner, Gergely; Plesz, Mónika; Jágerszki, Gyula; Fürjes, P.; Gyurcsányi, R.

In: Electrochemistry Communications, Vol. 71, 01.10.2016, p. 13-17.

Research output: Contribution to journalArticle

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