Na,K-ATPase receptor subunits α1, α2 and α3 mRNA in dilated cardiomyopathy

C. Sylven, E. Jansson, P. Sótónyi, F. Waagstein, M. Bronnegard

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Na,K-ATPase receptor density has been shown to be down-regulated with decreasing ejection fraction in patients with chronic heart failure. It was the aim of the present study to determine whether down-regulation is detected also at the mRNA level. Six donor hearts and six explanted hearts due to dilated cardiomyopathy (ejection fraction 23 ± 5%) were analyzed. RNA was extracted. Quantitative Na,K-ATPase receptor catalytic subunit α1, α2 and α3 mRNA expression was determined by solution hybridization. No cross-reactivity occurred between the three probes. α1 mRNA was expressed at about 5 and 10 times higher (p <0.001) concentrations than α2 and α3 mRNA, respectively, and α2 mRNA higher (p <0.001) than α3. There were no differences between right and left ventricles and between donor hearts and patients with dilated cardiomyopathy. In conclusion, Na,K-ATPase α1 mRNA is the predominant subunit expressed in human myocardium. Depressed ejection fraction in dilated cardiomyopathy is not associated with changed mRNA subunit expression. Documented downregulation of Na,K-ATPase activity, therefore, may be associated with the structural and membrane-related β subunit or posttransciptional modification of the catalytic subunits.

Original languageEnglish
Pages (from-to)907-909
Number of pages3
JournalBiological and Pharmaceutical Bulletin
Volume18
Issue number6
Publication statusPublished - 1995

Fingerprint

Dilated Cardiomyopathy
Messenger RNA
Heart Ventricles
Catalytic Domain
Down-Regulation
Tissue Donors
sodium-translocating ATPase
Myocardium
Heart Failure
RNA
Membranes

Keywords

  • Chronic heart failure
  • Messenger RNA
  • Myocardium
  • Na,K-ATPase

ASJC Scopus subject areas

  • Molecular Medicine
  • Pharmacology, Toxicology and Pharmaceutics(all)

Cite this

Na,K-ATPase receptor subunits α1, α2 and α3 mRNA in dilated cardiomyopathy. / Sylven, C.; Jansson, E.; Sótónyi, P.; Waagstein, F.; Bronnegard, M.

In: Biological and Pharmaceutical Bulletin, Vol. 18, No. 6, 1995, p. 907-909.

Research output: Contribution to journalArticle

Sylven, C, Jansson, E, Sótónyi, P, Waagstein, F & Bronnegard, M 1995, 'Na,K-ATPase receptor subunits α1, α2 and α3 mRNA in dilated cardiomyopathy', Biological and Pharmaceutical Bulletin, vol. 18, no. 6, pp. 907-909.
Sylven, C. ; Jansson, E. ; Sótónyi, P. ; Waagstein, F. ; Bronnegard, M. / Na,K-ATPase receptor subunits α1, α2 and α3 mRNA in dilated cardiomyopathy. In: Biological and Pharmaceutical Bulletin. 1995 ; Vol. 18, No. 6. pp. 907-909.
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AU - Bronnegard, M.

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