NADPH-diaphorase activity in the nervous system of the embryonic and juvenile pond snail, Lymnaea stagnalis

Z. Serfőző, K. Elekes, Vince Varga

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

Nicotinamide-adenine-dinucleotide-phosphate-diaphorase (NADPH-d) histochemistry has been applied in the present study to determine the distribution of putative nitric oxide (nitric oxide synthase)-producing cells during embryonic and early postembryonic development in the pond snail, Lymnaea stagnalis L., with special reference to the nervous system. The first NADPH-d-positive structures appear as early as 18% of development (E18, trochophore stage) and correspond to the pair of protonephridia. These structures later show disintegration, although after metamorphosis (E26 = 75%) staining of their individually spreading cells can be observed until hatching. Peripheral sensory neurons in the foot, mantle edge and lips, and their afferents projecting to the central nervous system reveal NADPH-d activity in the postmetamorphosis period (E25-E27 = E60%-E80%) of embryogenesis. After hatching (P1-P3), a number of stained sensory cells appear in the pharynx and esophagus. Some NADPH-d positive neuronal perikarya occur in the pedal and pleural ganglia, and a few weakly stained cells in the cerebral and buccal ganglia of juvenile snails. At the same time, a continuous bundle of reactive fibers is formed in the neuropil both through and through around the circumesophageal ganglion ring. The localization of NADPH-d activity in the developing nervous system of Lymnaea suggests that nitric oxide participates mainly in sensory processes. However, its role in specific intraganglionic integrative events cannot be excluded following embryonic metamorphosis.

Original languageEnglish
Pages (from-to)579-586
Number of pages8
JournalCell and Tissue Research
Volume292
Issue number3
DOIs
Publication statusPublished - 1998

Fingerprint

Lymnaea
NADPH Dehydrogenase
Snails
Neurology
Ponds
NADP
Nervous System
Ganglia
Foot
Nitric Oxide
Neuropil
Cheek
Disintegration
Sensory Receptor Cells
Lip
Pharynx
Nitric Oxide Synthase
Esophagus
Neurons
Embryonic Development

Keywords

  • Development, ontogenetic
  • Lymnaea stagnalis (Mollusca)
  • NADPH-diaphorase
  • Nitric oxide synthase

ASJC Scopus subject areas

  • Anatomy
  • Clinical Biochemistry
  • Cell Biology

Cite this

NADPH-diaphorase activity in the nervous system of the embryonic and juvenile pond snail, Lymnaea stagnalis. / Serfőző, Z.; Elekes, K.; Varga, Vince.

In: Cell and Tissue Research, Vol. 292, No. 3, 1998, p. 579-586.

Research output: Contribution to journalArticle

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