Na, K-ATpase Receptor Subunits α1, α2 and α3 mRNA in Dilated Cardiomyopathy

Christer Sylvén, Eva Jansson, Peter Sotonyi, Finn Waagstein, Mikael Brönnegärd

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Na, K-ATPase receptor density has been shown to be down-regulated with decreasing ejection fraction in patients with chronic heart failure. It was the aim of the present study to determine whether down-regulation is detected also at the mRNA level. Six donor hearts and six explanted hearts due to dilated cardiomyopathy (ejection fraction 23±5%) were analyzed. RNA was extracted. Quantitative Na, K-ATPase receptor catalytic subunit α1, α2 and α3 mRNA expression was determined by solution hybridization. No cross-reactivity occurred between the three probes. α1 mRNA was expressed at about 5 and 10 times higher (p<0.001) concentrations than α2 and α3 mRNA, respectively, and α2 mRNA higher (p<0.001) than α3. There were no differences between right and left ventricles and between donor hearts and patients with dilated cardiomyopathy. In conclusion, Na, K-ATPase α1 mRNA is the predominant subunit expressed in human myocardium. Depressed ejection fraction in dilated cardiomyopathy is not associated with changed mRNA subunit expression. Documented downregulation of Na, K-ATPase activity, therefore, may be associated with the structural and membrane-related β subunit or posttransciptional modification of the catalytic subunits.

Original languageEnglish
Pages (from-to)907-909
Number of pages3
JournalBiological and Pharmaceutical Bulletin
Volume18
Issue number6
DOIs
Publication statusPublished - Jan 1 1995

Keywords

  • K-ATPase
  • Na
  • chronic heart failure
  • messenger RNA
  • myocardium

ASJC Scopus subject areas

  • Pharmacology
  • Pharmaceutical Science

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