N-methyl-D-aspartate induces cortical hyperemia through cortical spreading depression-dependent and -independent mechanisms in rats

Laura Lenti, F. Domoki, Tamás Gáspár, James A. Snipes, F. Bari, David W. Busija

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Objective: N-methyl-d-aspartate (NMDA) is a powerful cerebrovascular dilator in vivo. Cortical spreading depression (CSD) has recently been shown to contribute to the pial arteriolar dilation in mice. Our main aim was to examine the participation of CSD in the overall cerebrovascular response to NMDA in the rat. Methods: Anesthetized Wistar rats (eight weeks old) were equipped with a closed cranial window to allow topical application of NMDA (10 ?5-10 ?3 M) to the parietal cortex. Cortical blood flow (CoBF) under and outside the cranial window was simultaneously monitored by using a two-channel laser-Doppler flowmeter. CSDs were detected by recording the changes in the cortical DC potential. Results: Concentrations of 10 ?4 and 10 ?3 M of NMDA evoked single CSDs associated with rapid, transient hyperemia, followed by a sustained, but reduced, increase in CoBF. The latency and magnitude of the CoBF responses were dose dependent. The higher dose resulted in shorter latency (100±5* vs. 146±11 seconds, *P

Original languageEnglish
Pages (from-to)629-639
Number of pages11
JournalMicrocirculation
Volume16
Issue number7
DOIs
Publication statusPublished - 2009

Fingerprint

Cortical Spreading Depression
Hyperemia
N-Methylaspartate
Aspartic Acid
Flowmeters
Parietal Lobe
Wistar Rats
Dilatation
Lasers

Keywords

  • AMPA
  • cerebral blood flow
  • glutamate
  • kainate
  • laser-Doppler flowmetry
  • piglet

ASJC Scopus subject areas

  • Physiology
  • Physiology (medical)
  • Molecular Biology
  • Cardiology and Cardiovascular Medicine
  • Medicine(all)

Cite this

N-methyl-D-aspartate induces cortical hyperemia through cortical spreading depression-dependent and -independent mechanisms in rats. / Lenti, Laura; Domoki, F.; Gáspár, Tamás; Snipes, James A.; Bari, F.; Busija, David W.

In: Microcirculation, Vol. 16, No. 7, 2009, p. 629-639.

Research output: Contribution to journalArticle

Lenti, Laura ; Domoki, F. ; Gáspár, Tamás ; Snipes, James A. ; Bari, F. ; Busija, David W. / N-methyl-D-aspartate induces cortical hyperemia through cortical spreading depression-dependent and -independent mechanisms in rats. In: Microcirculation. 2009 ; Vol. 16, No. 7. pp. 629-639.
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