N-methyl-D-aspartate-induced vasodilation is mediated by endothelium-independent nitric oxide release in piglets

F. Domoki, James V. Perciaccante, Katsuyoshi Shimizu, Michelle Puskar, David W. Busija, F. Bari

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

N-methyl-D-aspartate (NMDA) elicits pial arteriolar dilation that has been associated with neuronal nitric oxide (NO) production. However, endothelial factors or glial P-450 epoxygenase products may play a role. We tested whether NMDA-induced pial vasodilation 1) primarily involves NO diffusion from the parenchyma to the surface arterioles, 2) involves intact endothelial function, and 3) involves a miconazole-sensitive component. Arteriolar diameters were determined using closed cranial window-intravital microscopy in anesthetized piglets. NMDA (10-100 μM) elicited virtually identical dose-dependent dilations in paired arterioles (r = 0.94, n = 15). However, NMDA- but not bradykinin (BK)-induced dilations of arteriolar sections over large veins were reduced by 31 ± 1% (means ± SE, P <0.05, n = 4) compared with adjacent sections on the cortical surface. Also, 100 μM NMDA increased cerebrospinal fluid levels of NO metabolites from 3.7 ± 1.0 to 5.3 ± 1.2 μM (P <0.05, n = 6). Endothelial stunning by intracarotid injection of phorbol 12,13-dibutyrate did not affect NMDA-induced vasodilation but attenuated vascular responses to hypercapnia and BK by ∼70% (n = 7). Finally, miconazole (n = 6, 20 μM) pretreatment and coapplication with NMDA did not alter vascular responses to NMDA. In conclusion, NMDA appears to dilate pial arterioles exclusively through release and diffusion of NO from neurons to the pial surface in piglets.

Original languageEnglish
JournalAmerican Journal of Physiology - Heart and Circulatory Physiology
Volume282
Issue number4 51-4
Publication statusPublished - 2002

Fingerprint

N-Methylaspartate
Vasodilation
Endothelium
Nitric Oxide
Arterioles
Miconazole
Dilatation
Bradykinin
Blood Vessels
Phorbol 12,13-Dibutyrate
Hypercapnia
Neuroglia
Cerebrospinal Fluid
Veins
Neurons
Injections

Keywords

  • Bradykinin
  • Cerebral circulation
  • Miconazole
  • Pial arterioles

ASJC Scopus subject areas

  • Physiology

Cite this

N-methyl-D-aspartate-induced vasodilation is mediated by endothelium-independent nitric oxide release in piglets. / Domoki, F.; Perciaccante, James V.; Shimizu, Katsuyoshi; Puskar, Michelle; Busija, David W.; Bari, F.

In: American Journal of Physiology - Heart and Circulatory Physiology, Vol. 282, No. 4 51-4, 2002.

Research output: Contribution to journalArticle

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