N-glycosylation analysis of biopharmaceuticals by multicapillary gel electrophoresis: Generation and application of a new glucose unit database

Csenge Filep, Beata Borza, Gabor Jarvas, A. Guttman

Research output: Contribution to journalArticle

Abstract

As glycomics research is gaining momentum in the biopharmaceutical industry, there is an increasing need for reproducible high throughput glycoanalytical methods to monitor and characterize the N-glycosylation of therapeutic glycoproteins. Since the glycosylation pattern of glycobiotherapeutics influences their important biological functions, approaches to comprehensively analyze these complex molecules is of high importance. This paper reports on the use of multicapillary gel electrophoresis in high throughput analysis of fluorophore labeled partitioned N-glycan libraries to generate a new glucose unit database that was consequently applied to identify the carbohydrate structures of two high profile biopharmaceuticals, adalimumab and etanercept.

Original languageEnglish
Article number112892
JournalJournal of Pharmaceutical and Biomedical Analysis
Volume178
DOIs
Publication statusPublished - Jan 30 2020

Fingerprint

Glycosylation
Capillary electrophoresis
Capillary Electrophoresis
Electrophoresis
Polysaccharides
Gels
Throughput
Glycomics
Databases
Glucose
Fluorophores
Glycoproteins
Momentum
Industry
Carbohydrates
Molecules
Research
Therapeutics
Adalimumab
Etanercept

Keywords

  • Biotherapeutics
  • Capillary electrophoresis
  • Glycans

ASJC Scopus subject areas

  • Analytical Chemistry
  • Pharmaceutical Science
  • Drug Discovery
  • Spectroscopy
  • Clinical Biochemistry

Cite this

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AU - Guttman, A.

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AB - As glycomics research is gaining momentum in the biopharmaceutical industry, there is an increasing need for reproducible high throughput glycoanalytical methods to monitor and characterize the N-glycosylation of therapeutic glycoproteins. Since the glycosylation pattern of glycobiotherapeutics influences their important biological functions, approaches to comprehensively analyze these complex molecules is of high importance. This paper reports on the use of multicapillary gel electrophoresis in high throughput analysis of fluorophore labeled partitioned N-glycan libraries to generate a new glucose unit database that was consequently applied to identify the carbohydrate structures of two high profile biopharmaceuticals, adalimumab and etanercept.

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