N-Deacetylation of Sinorhizobium meliloti Nod factors increases their stability in the Medicago sativa rhizosphere and decreases their biological activity

Christian Staehelin, Michael Schultze, Ken Tokuyasu, Véréna Poinsot, Jean Claude Promé, Éva Kondorosi, Ádám Kondorosi

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Nod factors excreted by rhizobia are signal molecules that consist of a chitin oligomer backbone linked with a fatty acid at the nonreducing end. Modifications of the Nod factor structures influence their stability in the rhizosphere and their biological activity. To test the function of N-acetyl groups in Nod factors, NodSm-IV(C(16:2),S) from Sinorhizobium meliloti was enzymatically N-deacetylated in vitro with purified chitin deacetylase from Colletotrichum lindemuthianum. A family of partially and completely deacetylated derivatives was produced and purified. The most abundant chemical structures identified by mass spectrometry were GlcN(C(16:2))-GlcNAc-GlcNH2-GlcNAc(OH)(S), GlcN(C(16:2))GlcNAc-GlcNH2-GlcNH2(OH)(S), and GlcN(C(16:2))GlcNH2-GlcNH2-GlcNH2(OH)(S). In contrast to NodSm-IV(C(16:2),S), the purified N-deacetylated derivatives were stable in the rhizosphere of Medicago sativa, indicating that the N-acetyl groups make the carbohydrate moiety of Nod factors accessible for glycosyl hydrolases of the host plant. The N-deacetylated derivatives displayed only a low level of activity in inducing root hair deformation. Furthermore, the N-deacetylated molecules were not able to stimulate Nod factor degradation by M. sativa roots, a response elicited by active Nod factors. These data show that N-acetyl groups of Nod factors are required for biological activity.

Original languageEnglish
Pages (from-to)72-79
Number of pages8
JournalMolecular Plant-Microbe Interactions
Volume13
Issue number1
DOIs
Publication statusPublished - Jan 2000

Keywords

  • Lipo-chitooligosaccharides

ASJC Scopus subject areas

  • Physiology
  • Agronomy and Crop Science

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