N-acetyl-L-arginine ethyl ester synthesis catalysed by bovine trypsin in organic media

L. M. Simon, M. Kotormán, K. Maráczi, K. László

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

The bovine trypsin-catalysed synthesis of N-acetyl-L-arginine ethyl ester from N-acetyl-L-arginine and ethanol was studied in various organic solvents (dimethyl sulfoxide, dioxane, dimethylformamide, acetonitrile, acetone, tetrahydrofuran, chloroform, toluene, carbon tetrachloride, cyclohexane and n-hexane). The highest yield was achieved in acetonitrile after incubation for 6 or 24 h. The optimal conditions for ester synthesis in acetonitrile for 6 h were as follows: 5.0 mM N-acetyl-L-arginine, 10.0 M ethanol, 7.2 mg trypsin, 2.87% water, total volume 10.3 ml, pH 7.0 and 30°C. The hydrolytic activity of trypsin was determined after incubation for 6 days, when 87.7% of the original activity remained, suggesting that acetonitrile caused little inactivation of the enzyme. The synthetic reaction resulted in a maximal 79.3% conversion under optimized conditions after incubation for 48 h. (C) 2000 Elsevier Science B.V.

Original languageEnglish
Pages (from-to)565-570
Number of pages6
JournalJournal of Molecular Catalysis B: Enzymatic
Volume10
Issue number6
Publication statusPublished - Nov 1 2000

Fingerprint

Arginine
Acetonitrile
Trypsin
Esters
Ethanol
Dimethylformamide
Carbon tetrachloride
Dimethyl sulfoxide
Carbon Tetrachloride
Toluene
Cyclohexane
Chloroform
Chlorine compounds
Acetone
Dimethyl Sulfoxide
Hexane
Organic solvents
Enzymes
N-acetyl-L-arginine
acetonitrile

Keywords

  • Acetonitrile
  • Bovine trypsin
  • N-acetyl-L-arginine ethyl ester synthesis
  • Optimization

ASJC Scopus subject areas

  • Biochemistry
  • Catalysis
  • Process Chemistry and Technology

Cite this

N-acetyl-L-arginine ethyl ester synthesis catalysed by bovine trypsin in organic media. / Simon, L. M.; Kotormán, M.; Maráczi, K.; László, K.

In: Journal of Molecular Catalysis B: Enzymatic, Vol. 10, No. 6, 01.11.2000, p. 565-570.

Research output: Contribution to journalArticle

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