Mytilus inhibitory peptide (MIP) induces a Na+-activated K+-current in snail neurons

T. Kiss, Y. Fujisawa, Z. Laszlo, Y. Muneoka

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Two microelectrode voltage-clamp and single-channel recordings were performed on D-cluster neurons of snail right parietal ganglion in order to study the properties of MIP-activated potassium current. It was found that the octapeptide member of the MIP-family, ASHIPRFVa elicits an outward current, which possesses all the properties characteristic for the hexapeptide(s) inward membrane response. The main component of the peptide elicited response is highly [K+](o) dependent, however the response was attenauted in Na-free extracellular saline. The peptide elicited response was mimicked by raising the [Na+](i) by pressure injection of Na+ into the cell. Single channel recordings indicated that MIP-induced outward K-current is Na-dependent. The probability to find a channel in open state increases with increasing intracellular Na+-concentration. Excised inside-out patches obtained from D-neurons contained I(K(Na)) channels could be activated by exposure of the cytoplasmic face of the patch membrane to 40 mM Na+, and 40 mM Li+, as well. The single channel current amplitude at -60 mV is 15 pA and the single channel conductance is 212 pS between -80 and 0 mV. It was concluded that MIP's activate a novel type of K+-current in the snail neurons. This current is the Na-activated K+-current. The single channel properties of the MIP activated channel is in concert with I(K(Na)) data obtained on different vertebrate and invertebrate preparations.

Original languageEnglish
Pages (from-to)133-145
Number of pages13
JournalActa Biologica Hungarica
Volume51
Issue number2-4
Publication statusPublished - 2000

Fingerprint

Mytilus
Snails
peptide
snail
Neurons
snails
neurons
peptides
Membranes
Peptides
Microelectrodes
Clamping devices
Invertebrates
Ganglia
Vertebrates
Potassium
membrane
Pressure
Injections
Mytilus inhibitory peptide

Keywords

  • Cation-activated potassium channels
  • Invertebrates
  • Neuron
  • Neuropeptide
  • Snail

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)

Cite this

Mytilus inhibitory peptide (MIP) induces a Na+-activated K+-current in snail neurons. / Kiss, T.; Fujisawa, Y.; Laszlo, Z.; Muneoka, Y.

In: Acta Biologica Hungarica, Vol. 51, No. 2-4, 2000, p. 133-145.

Research output: Contribution to journalArticle

Kiss, T, Fujisawa, Y, Laszlo, Z & Muneoka, Y 2000, 'Mytilus inhibitory peptide (MIP) induces a Na+-activated K+-current in snail neurons', Acta Biologica Hungarica, vol. 51, no. 2-4, pp. 133-145.
Kiss, T. ; Fujisawa, Y. ; Laszlo, Z. ; Muneoka, Y. / Mytilus inhibitory peptide (MIP) induces a Na+-activated K+-current in snail neurons. In: Acta Biologica Hungarica. 2000 ; Vol. 51, No. 2-4. pp. 133-145.
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