Myocardial salvage by l-o-hexadecyl-sn-glycerol: Possible role of peroxisomal dysfunction in ischemia reperfusion injury

Nilanjana Maulik, A. Tósaki, Richard M. Engelman, Gerald A. Cordis, Dipak K. Das

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

A recent study demonstrated biochemical and structural alterations of peroxisomes in rat kidney after ischemia/reperfusion. We examined whether peroxisomes play any role in the pathophysiology of myocardial ischemia/reperfusion injury. Isolated perfused rat heart was made ischemie for 30 min by terminating coronary flow (CF), followed by 30-min reperfusion. Experiments were divided into two groups; the experimental group received 1-O-hexadecyl-Sn-glycerol (chimyl alcohol) (25, 50, and 100 μÌ) before ischemia, and the control group received an equivalent amount of saline. Two of the experimental groups (50 and 100 μM) demonstrated improved postischemic myocardial performance, as demonstrated by accelerated recovery in left ventricular developed pressure (LVDP) and CF, as well as reduction in the incidence of ventricular fibrillation (VF). However, because the heart rate (HR) was significantly reduced in the 100-μÌ chimyl alcohol group, subsequent studies were performed with 50 μÌ chimyl alcohol as the optimal dose. Chimyl alcohol (50 μÌ) also reduced cellular injury, as evidenced by reduced creatine kinase (CK) release, and decreased development of oxidative stress, as evidenced by reduced formation of malonaldehyde (MDA). Peroxisomal catalase activity was decreased in the control group after ischemia/reperfusion, and chimyl alcohol treatment restored the activity of the enzyme. Our results indicate that chimyl alcohol, a precursor of ether-linked phosphoglyceride biosynthesis, can reduce myocardial ischemia/reperfusion injury, possibly by restoring catalase activity and reducing oxidative stress through synthesis of ether lipids, suggesting a possible role of peroxisomal disorder in ischemia/reperfusion injury.

Original languageEnglish
Pages (from-to)486-492
Number of pages7
JournalJournal of Cardiovascular Pharmacology
Volume24
Issue number3
Publication statusPublished - 1994

Fingerprint

Reperfusion Injury
Glycerol
Reperfusion
Myocardial Reperfusion Injury
Peroxisomes
Ischemia
Ether
Catalase
Myocardial Ischemia
Oxidative Stress
Peroxisomal Disorders
Glycerophospholipids
Control Groups
Ventricular Fibrillation
Ventricular Pressure
Creatine Kinase
Malondialdehyde
chimyl alcohol
Heart Rate
Kidney

Keywords

  • Chimyl alcohol
  • Heart
  • Ischemia
  • Peroxisome
  • Reperfusion
  • Reperfusion injury

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine
  • Pharmacology

Cite this

Myocardial salvage by l-o-hexadecyl-sn-glycerol : Possible role of peroxisomal dysfunction in ischemia reperfusion injury. / Maulik, Nilanjana; Tósaki, A.; Engelman, Richard M.; Cordis, Gerald A.; Das, Dipak K.

In: Journal of Cardiovascular Pharmacology, Vol. 24, No. 3, 1994, p. 486-492.

Research output: Contribution to journalArticle

Maulik, Nilanjana ; Tósaki, A. ; Engelman, Richard M. ; Cordis, Gerald A. ; Das, Dipak K. / Myocardial salvage by l-o-hexadecyl-sn-glycerol : Possible role of peroxisomal dysfunction in ischemia reperfusion injury. In: Journal of Cardiovascular Pharmacology. 1994 ; Vol. 24, No. 3. pp. 486-492.
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