Myelin basic protein, an autoantigen in multiple sclerosis, is selectively processed by human trypsin 4

Péter Medveczky, József Antal, András Patthy, Katalin Kékesi, Gábor Juhász, László Szilágyi, László Gráf

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

Demyelination, the proteolytic degradation of the major membrane protein in central nervous system, myelin, is involved in many neurodegenerative diseases. In the present in vitro study the proteolytic actions of calpain, human trypsin 1 and human trypsin 4 were compared on lipid bound and free human myelin basic proteins as substrates. The fragments formed were identified by using N-terminal amino acid sequencing and mass spectrometry. The analysis of the degradation products showed that of these three proteases human trypsin 4 cleaved myelin basic protein most specifically. It selectively cleaves the Arg79-Thr80 and Arg97-Thr98 peptide bonds in the lipid bound form of human myelin basic protein. Based on this information we synthesized peptide IVTPRTPPPSQ that corresponds to sequence region 93-103 of myelin basic protein and contains one of its two trypsin 4 cleavage sites, Arg97-Thr98. Studies on the hydrolysis of this synthetic peptide by trypsin 4 have confirmed that the Arg97-Thr98 peptide bond is highly susceptible to trypsin 4. What may lend biological interest to this finding is that the major autoantibodies found in patients with multiple sclerosis recognize sequence 85-96 of the protein. Our results suggest that human trypsin 4 may be one of the candidate proteases involved in the pathomechanism of multiple sclerosis.

Original languageEnglish
Pages (from-to)545-552
Number of pages8
JournalFEBS letters
Volume580
Issue number2
DOIs
Publication statusPublished - Jan 23 2006

Keywords

  • Autoimmune peptide
  • Central nervous system
  • Myelin degradation
  • Proteolysis
  • Serine protease
  • Trypsin

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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