Mycotoxin biosensor based on optical planar waveguide

Ali Al-Jawdah, Alexei Nabok, Radhyah Jarrah, Alan Holloway, Anna Tsargorodska, Eszter Takacs, Andras Szekacs

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

The research aim of this work is to develop a simple and highly sensitive optical biosensor for detection of mycotoxins. This sensor is built on a planar waveguide operating on the polarization interferometry principle, i.e., detecting a phase shift between p- and s-components of polarized light developed during the binding of analyte molecules. The operation of the proposed sensor is similar to that of a Mach–Zehnder interferometer, while its design is much simpler and it does not require splitting the waveguide into two arms. The refractive index sensitivity of the polarization interferometer sensor was in the range of 5200 radians per refractive index unit (RIU). Several tests were conducted to detect ochratoxin A (OTA) at different concentrations in direct immunoassay with specific antibodies immobilized in the sensing window. The lowest concentration of OTA of 0.01 ng/mL caused a phase shift of nearly one period. The results obtained prove high sensitivity of the sensors, which are capable of detecting even lower concentrations of mycotoxins at the ppt (part-per-trillion) level.

Original languageEnglish
Article number272
JournalToxins
Volume10
Issue number7
DOIs
Publication statusPublished - Jul 3 2018

Keywords

  • Mycotoxins
  • Optical biosensor
  • Planar waveguide
  • Polarization interferometer
  • Refractive index sensitivity

ASJC Scopus subject areas

  • Toxicology
  • Health, Toxicology and Mutagenesis

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  • Cite this

    Al-Jawdah, A., Nabok, A., Jarrah, R., Holloway, A., Tsargorodska, A., Takacs, E., & Szekacs, A. (2018). Mycotoxin biosensor based on optical planar waveguide. Toxins, 10(7), [272]. https://doi.org/10.3390/toxins10070272