Papaver somniferum produces secondary metabolites, which have important roles in their self-defence processes, in plant biochemistry and in allelochemistry. We can see that different stress effects change the quantity of alkaloids. The object of the experiments is, in what manner changes the content of alkaloids of poppy in case of irregular stress effects. Papaver somniferum (cv. 'Kék Duna', Budakalász) plants were grown for 2 months from seeds in quartz-sand (in natural light, temperature: 24-28 °C, in Knop's nutritive solution). In this paper we studied the alkaloid of poppy treated with two kind of stress factors: mycotoxin and drought, respectively. Both the quantity and the spectrum of alkaloids were measured after different separation procedures. Thin layer chromatography (TLC and HPTLC) and high performance liquid chromatography (HPLC) were applied. Content of the level of formaldehyde (HCHO) also increases in plants with different stress effects. Our presupposition is that the formation of methyl groups of poppy alkaloids takes place through HCHO. It gave us an opportunity to examine changing of formaldehyde (HCHO) level in biotic and abiotic stress situation. Formaldehyde in dimedone adduct form can be detected in injured tissues of Papaver somniferum. As a consequence, the stress effects can be detected in poppy plants by two kinds of method. At first we measured content of alkaloids. Drought stress produced a higher level of the alkaloids, but the mycotoxin stress did not show significant results.
- Morphine alkaloids
- Stress effects
ASJC Scopus subject areas
- Ecology, Evolution, Behavior and Systematics
- Plant Science