Mycobacterial RNase E-associated proteins

Lorand Kovacs, Agnes Csanadi, Klara Megyeri, Vladimir R. Kaberdin, A. Miczák

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

RNase E and its complex with other proteins ('degradosome') play an important role in RNA processing and decay in Escherichia coli and in many other bacteria. To identify the proteins which can potentially interact with this enzyme in mycobacteria, Mycobacterium tuberculosis H37Rv RNase E was cloned and expressed as a 6HisFLAG-tagged fusion protein. Analysis of the mycobacterial RNase E overexpressed and purified from M. bovis BCG revealed the presence of GroEL and two other copurified proteins, products of the Mb1721 (inorganic polyphosphate/ATP-NAD kinase) and Mb0825c (acetyltransferase) genes. Identical copies of these two genes can be found in M. tuberculosis H37Rv.

Original languageEnglish
Pages (from-to)1003-1007
Number of pages5
JournalMicrobiology and Immunology
Volume49
Issue number11
Publication statusPublished - 2005

Fingerprint

Mycobacterium tuberculosis
Proteins
Acetyltransferases
RNA Stability
Mycobacterium
Mycobacterium bovis
Genes
Adenosine Triphosphate
Escherichia coli
Bacteria
ribonuclease E
Enzymes
degradosome
polyphosphate NAD-kinase

Keywords

  • Degradosome
  • Mycobacterium
  • RNA metabolism
  • RNase E

ASJC Scopus subject areas

  • Immunology and Microbiology(all)
  • Microbiology (medical)
  • Microbiology

Cite this

Kovacs, L., Csanadi, A., Megyeri, K., Kaberdin, V. R., & Miczák, A. (2005). Mycobacterial RNase E-associated proteins. Microbiology and Immunology, 49(11), 1003-1007.

Mycobacterial RNase E-associated proteins. / Kovacs, Lorand; Csanadi, Agnes; Megyeri, Klara; Kaberdin, Vladimir R.; Miczák, A.

In: Microbiology and Immunology, Vol. 49, No. 11, 2005, p. 1003-1007.

Research output: Contribution to journalArticle

Kovacs, L, Csanadi, A, Megyeri, K, Kaberdin, VR & Miczák, A 2005, 'Mycobacterial RNase E-associated proteins', Microbiology and Immunology, vol. 49, no. 11, pp. 1003-1007.
Kovacs L, Csanadi A, Megyeri K, Kaberdin VR, Miczák A. Mycobacterial RNase E-associated proteins. Microbiology and Immunology. 2005;49(11):1003-1007.
Kovacs, Lorand ; Csanadi, Agnes ; Megyeri, Klara ; Kaberdin, Vladimir R. ; Miczák, A. / Mycobacterial RNase E-associated proteins. In: Microbiology and Immunology. 2005 ; Vol. 49, No. 11. pp. 1003-1007.
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