Mutations in the protein phosphatase 1 gene at 87B can differentially affect suppression of position-effect variegation and mitosis in Drosophila melanogaster

K. Baksa, H. Morawietz, V. Dombrádi, M. Axton, H. Taubert, G. Szabo, I. Torok, A. Udvardy, H. Gyurkovics, B. Szoor, D. Glover, G. Reuter, J. Gausz

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Abstract

The suppressor of position effect variegation (PEV) locus Su-var(3)6 maps to 87B5-10. The breakpoints of deficiencies that define this interval have been placed on a 250-kb molecular map of the region. The locus is allelic to the ck19 complementation group previously shown to encode a type 1 serine- threonine protein phosphatase (PP1) catalytic subunit. When introduced into flies by P element-mediated transformation, a 5.8-kb genomic fragment carrying this gene overcomes the suppressor phenotype of Su-var(3)601 and recessive lethality of all mutations of the locus. Four of the mutant alleles at the locus show a broad correlation between high levels of suppression of PEV, a high frequency of aberrant mitosis and low PP1 activity in larval extracts. However, some alleles with low PP1 activity show weak suppression of PEV with a high frequency of abnormal mitosis, whereas others show strong suppression of PEV with normal mitosis. The basis for these disparate phenotypes is discussed.

Original languageEnglish
Pages (from-to)117-125
Number of pages9
JournalGenetics
Volume135
Issue number1
Publication statusPublished - 1993

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Chromosomal Position Effects
Protein Phosphatase 1
Drosophila melanogaster
Mitosis
Mutation
Genes
Alleles
Suppressor Genes
Phenotype
Diptera
Catalytic Domain

ASJC Scopus subject areas

  • Genetics
  • Genetics(clinical)

Cite this

Mutations in the protein phosphatase 1 gene at 87B can differentially affect suppression of position-effect variegation and mitosis in Drosophila melanogaster. / Baksa, K.; Morawietz, H.; Dombrádi, V.; Axton, M.; Taubert, H.; Szabo, G.; Torok, I.; Udvardy, A.; Gyurkovics, H.; Szoor, B.; Glover, D.; Reuter, G.; Gausz, J.

In: Genetics, Vol. 135, No. 1, 1993, p. 117-125.

Research output: Contribution to journalArticle

Baksa, K, Morawietz, H, Dombrádi, V, Axton, M, Taubert, H, Szabo, G, Torok, I, Udvardy, A, Gyurkovics, H, Szoor, B, Glover, D, Reuter, G & Gausz, J 1993, 'Mutations in the protein phosphatase 1 gene at 87B can differentially affect suppression of position-effect variegation and mitosis in Drosophila melanogaster', Genetics, vol. 135, no. 1, pp. 117-125.
Baksa, K. ; Morawietz, H. ; Dombrádi, V. ; Axton, M. ; Taubert, H. ; Szabo, G. ; Torok, I. ; Udvardy, A. ; Gyurkovics, H. ; Szoor, B. ; Glover, D. ; Reuter, G. ; Gausz, J. / Mutations in the protein phosphatase 1 gene at 87B can differentially affect suppression of position-effect variegation and mitosis in Drosophila melanogaster. In: Genetics. 1993 ; Vol. 135, No. 1. pp. 117-125.
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AU - Morawietz, H.

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AU - Axton, M.

AU - Taubert, H.

AU - Szabo, G.

AU - Torok, I.

AU - Udvardy, A.

AU - Gyurkovics, H.

AU - Szoor, B.

AU - Glover, D.

AU - Reuter, G.

AU - Gausz, J.

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