Mutational analysis of the HTLV-I trans-activator, Tax

András Heder, Saowakon Paca-Uccaralertkun, Imre Boros

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

The gene coding for the trans-activating factor (Tax) of the human T-cell leukemia virus, type I (HTLV-I) was mutagenized in vitro using oligonucleotide-directed mutagenesis and recombinant DNA techniques. All except one of the mutagenized tax constructs failed to trans-activate the HTLV-I LTR in a eukaryotic test system. Moreover, negative Tax mutant Arg-39→Gly was found to be trans-dominant. This observation suggests that Tax contains distinct functional domains mediating different interactions of the protein in the process of trans-activation.

Original languageEnglish
Pages (from-to)210-212
Number of pages3
JournalFEBS letters
Volume292
Issue number1-2
DOIs
Publication statusPublished - Nov 4 1991

Keywords

  • CAT assay
  • Human T-cell leukemia virus
  • Oligonucleotide-directed mutagenesis
  • Trans-activation
  • Trans-dominant mutant

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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