Mutational analysis of the HTLV-I trans-activator, Tax

András Heder, Saowakon Paca-Uccaralertkun, I. Boros

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

The gene coding for the trans-activating factor (Tax) of the human T-cell leukemia virus, type I (HTLV-I) was mutagenized in vitro using oligonucleotide-directed mutagenesis and recombinant DNA techniques. All except one of the mutagenized tax constructs failed to trans-activate the HTLV-I LTR in a eukaryotic test system. Moreover, negative Tax mutant Arg-39→Gly was found to be trans-dominant. This observation suggests that Tax contains distinct functional domains mediating different interactions of the protein in the process of trans-activation.

Original languageEnglish
Pages (from-to)210-212
Number of pages3
JournalFEBS Letters
Volume292
Issue number1-2
DOIs
Publication statusPublished - Nov 4 1991

Fingerprint

Human T-lymphotropic virus 1
Trans-Activators
T-cells
Taxation
Viruses
Recombinant DNA
Site-Directed Mutagenesis
Mutagenesis
Oligonucleotides
Genes
Proteins
Chemical activation
In Vitro Techniques

Keywords

  • CAT assay
  • Human T-cell leukemia virus
  • Oligonucleotide-directed mutagenesis
  • Trans-activation
  • Trans-dominant mutant

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Mutational analysis of the HTLV-I trans-activator, Tax. / Heder, András; Paca-Uccaralertkun, Saowakon; Boros, I.

In: FEBS Letters, Vol. 292, No. 1-2, 04.11.1991, p. 210-212.

Research output: Contribution to journalArticle

Heder, András ; Paca-Uccaralertkun, Saowakon ; Boros, I. / Mutational analysis of the HTLV-I trans-activator, Tax. In: FEBS Letters. 1991 ; Vol. 292, No. 1-2. pp. 210-212.
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