Mutational analysis of IgVH and BCL-6 genes suggests thymic B-cells origin of mediastinal (Thymic) B-cell lymphoma

B. Csernus, Botond Timár, Zsolt Fülöp, Ágnes Bognár, Ágota Szepesi, Terézia László, Pál Jáksó, Roger Warnke, L. Kópper, A. Matolcsy

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Mediastinal (thymic) large B-cell lymphoma (MBL) has been defined as a subtype of diffuse large B-cell lymphoma (DLBL) arising in the mediastinum with characteristic clinicopathological features. It has been postulated that MBL arise from non-circulating thymic B-cells and represent a distinct lymphoma entity, however, the histogenesis of the disease is not yet fully understood. In order to clarify the histogenetic derivation of MBL and to determine the relationship of MBL to thymic B-cells we have analyzed the nucleic acid sequences of immunoglobulin (Ig) heavy chain variable region (VH) and 5′ noncoding region of BCL-6 genes in normal thymic B-cells and six cases of MBL. Thymic B-cells and tumor cells of MBLs displayed hypermutated VH and/or BCL-6 genes but intraclonal divergence did not associate with these mutations. Since somatic mutations of the IgVH and BCL-6 genes are histogenetic markers of B-cell transit through the germinal centre (GC), these results suggest that both thymic B-cells and MBLs derived from GC or an equivalent environment where B-cells underwent somatic hypermutation. The similar pattern of mutations of IgVH and BCL-6 genes found in thymic B-cells and MBLs further supports the theory that MBLs originate from thymic B-cells.

Original languageEnglish
Pages (from-to)2105-2110
Number of pages6
JournalLeukemia and Lymphoma
Volume45
Issue number10
DOIs
Publication statusPublished - Oct 2004

Fingerprint

B-Cell Lymphoma
B-Lymphocytes
Genes
Germinal Center
Mutation
Immunoglobulin Heavy Chains
Lymphoma, Large B-Cell, Diffuse
Mediastinum
Nucleic Acids
Lymphoma

Keywords

  • BCL-6 gene
  • IgV gene
  • Mediastinal large B-cell lymphoma
  • Thymic B-cell

ASJC Scopus subject areas

  • Hematology
  • Oncology
  • Cancer Research

Cite this

Mutational analysis of IgVH and BCL-6 genes suggests thymic B-cells origin of mediastinal (Thymic) B-cell lymphoma. / Csernus, B.; Timár, Botond; Fülöp, Zsolt; Bognár, Ágnes; Szepesi, Ágota; László, Terézia; Jáksó, Pál; Warnke, Roger; Kópper, L.; Matolcsy, A.

In: Leukemia and Lymphoma, Vol. 45, No. 10, 10.2004, p. 2105-2110.

Research output: Contribution to journalArticle

Csernus, B. ; Timár, Botond ; Fülöp, Zsolt ; Bognár, Ágnes ; Szepesi, Ágota ; László, Terézia ; Jáksó, Pál ; Warnke, Roger ; Kópper, L. ; Matolcsy, A. / Mutational analysis of IgVH and BCL-6 genes suggests thymic B-cells origin of mediastinal (Thymic) B-cell lymphoma. In: Leukemia and Lymphoma. 2004 ; Vol. 45, No. 10. pp. 2105-2110.
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AU - Bognár, Ágnes

AU - Szepesi, Ágota

AU - László, Terézia

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