Mutation of a gene for a Drosophila kinesin-like protein, Klp38B, leads to failure of cytokinesis

Hiroyuki Ohkura, Tibor Török, Gabriella Tick, Jörg Hoheisel, I. Kiss, David M. Glover

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

Mutations in a gene (Klp38B) encoding a novel kinesin-like protein in Drosophila melanogaster lead to the formation of polyploid cells in the larval central nervous system and in the follicle cells of adult egg chambers. Some homozygous mutants survive to adulthood and also exhibit morphological defects indicative of abnormal cell cycle progression, including rough eyes, missing bristles, and abnormal abdominal cuticles. In larval brains, there is no accumulation of mitotic cells and the frequency of anaphase figures is comparable to wild type, suggesting that nuclear division is not affected. Such brains contain polyploid cells with metaphase and anaphase chromosomes associated with bipolar spindles. Such spindles have a number of unseparated centrosomes at their poles reflecting the degree of polyploidy of the cell. Follicle cells frequently contain two nuclei of roughly equal size. Taken together, we conclude that these Klp38B mutations lead to a failure of cytokinesis resulting in polyploidy, and discuss whether or not this is a direct effect of the mutation.

Original languageEnglish
Pages (from-to)945-954
Number of pages10
JournalJournal of Cell Science
Volume110
Issue number8
Publication statusPublished - 1997

Fingerprint

Kinesin
Polyploidy
Cytokinesis
Drosophila
Anaphase
Mutation
Genes
Proteins
Cell Nucleus Division
Drosophila Proteins
Centrosome
Brain
Metaphase
Ovum
Cell Cycle
Central Nervous System
Chromosomes

Keywords

  • Cytokinesis
  • Drosophila
  • Kinesin
  • Klp38B

ASJC Scopus subject areas

  • Cell Biology

Cite this

Ohkura, H., Török, T., Tick, G., Hoheisel, J., Kiss, I., & Glover, D. M. (1997). Mutation of a gene for a Drosophila kinesin-like protein, Klp38B, leads to failure of cytokinesis. Journal of Cell Science, 110(8), 945-954.

Mutation of a gene for a Drosophila kinesin-like protein, Klp38B, leads to failure of cytokinesis. / Ohkura, Hiroyuki; Török, Tibor; Tick, Gabriella; Hoheisel, Jörg; Kiss, I.; Glover, David M.

In: Journal of Cell Science, Vol. 110, No. 8, 1997, p. 945-954.

Research output: Contribution to journalArticle

Ohkura, H, Török, T, Tick, G, Hoheisel, J, Kiss, I & Glover, DM 1997, 'Mutation of a gene for a Drosophila kinesin-like protein, Klp38B, leads to failure of cytokinesis', Journal of Cell Science, vol. 110, no. 8, pp. 945-954.
Ohkura, Hiroyuki ; Török, Tibor ; Tick, Gabriella ; Hoheisel, Jörg ; Kiss, I. ; Glover, David M. / Mutation of a gene for a Drosophila kinesin-like protein, Klp38B, leads to failure of cytokinesis. In: Journal of Cell Science. 1997 ; Vol. 110, No. 8. pp. 945-954.
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