Mutation in afsR Leads to A-Factor Deficiency in Streptomyces griseus B2682

Melinda Szilágyi, Éva Márton, Dávid Lukács, Zsuzsanna Birkó, Z. Kele, Sándor Biró

Research output: Contribution to journalArticle

Abstract

Background/Aims: A-factor, a γ-butyrolactone autoregulator, in Streptomyces griseus is involved in the regulation of differentiation and antibiotic production. Here we studied the S. griseus B2682-AFN (A-factor negative) bald mutant that harbors a nonsense mutation in the afsR gene encoding a pleiotropic regulator. Our aim was to prove that this mutation is the cause of the A-factor deficiency in AFN. We also studied whether AfsR regulates A-factor production by AfsA, which is supposed to be the only specific key enzyme in A-factor biosynthesis. Methods: Wild afsR was cloned to the pHJL401 shuttle vector and was transformed to the S. griseus AFN and B2682 strains. During phenotypic characterization, sporulation, antibiotic, protease, A-factor, and AfsA protein production were studied. Results: Transformation of AFN by a wild afsR restored its phenotype including sporulation, antibiotic, extracellular protease, and A-factor production. Introduction of afsR to the B2682 wild-type strain resulted in antibiotic and extracellular protease overproduction that was accompanied with an elevated A-factor level. AfsA was detected both in AFN and B2682. Conclusions: AfsR has an effect on the regulation of A-factor production in S. griseus. The presence of AfsA is not sufficient for normal A-factor production. AfsR regulates A-factor biosynthesis independently of AfsA.

Original languageEnglish
Pages (from-to)216-224
Number of pages9
JournalJournal of Molecular Microbiology and Biotechnology
DOIs
Publication statusPublished - Jan 1 2019

Fingerprint

Streptomyces griseus
Mutation
Anti-Bacterial Agents
Peptide Hydrolases
A-factor (Streptomyces)
Genetic Vectors
Nonsense Codon
Phenotype

Keywords

  • A-factor
  • AfsA
  • AfsR
  • Antibiotics
  • Differentiation
  • Streptomyces genetics and regulation
  • Streptomyces griseus

ASJC Scopus subject areas

  • Biotechnology
  • Microbiology
  • Applied Microbiology and Biotechnology
  • Molecular Biology

Cite this

Mutation in afsR Leads to A-Factor Deficiency in Streptomyces griseus B2682. / Szilágyi, Melinda; Márton, Éva; Lukács, Dávid; Birkó, Zsuzsanna; Kele, Z.; Biró, Sándor.

In: Journal of Molecular Microbiology and Biotechnology, 01.01.2019, p. 216-224.

Research output: Contribution to journalArticle

Szilágyi, Melinda ; Márton, Éva ; Lukács, Dávid ; Birkó, Zsuzsanna ; Kele, Z. ; Biró, Sándor. / Mutation in afsR Leads to A-Factor Deficiency in Streptomyces griseus B2682. In: Journal of Molecular Microbiology and Biotechnology. 2019 ; pp. 216-224.
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