Muscarinic M1 and M3 receptors are present and increase intracellular calcium in adult rat anterior pituitary gland

Ildikó Pintér, Georg Moszkovszkin, Zsolt Némethy, G. Makara

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Physiological and biochemical evidence indicates the existence of functional muscarinic cholinergic receptors in the anterior pituitary. The selectivity of these receptors has been characterised by studying the binding of [3H]quinuclidinyl benzilate ([3H]QNB) and [3H]diphenyl-acetoxy-N- methyl-piperidine ([3H]4-DAMP) in membrane preparation of male rat anterior pituitary at 25°C. Competition experiments with receptor selective muscarinic antagonists were used to characterise specific selective muscarinic receptor binding. Both [3H]QNB and [3H]4-DAMP bound to anterior pituitary membranes at low concentrations, binding was saturable and was potently displaced by 4-DAMP (M1, M3 subtypes selective antagonist) > atropine (general) > pirenzepine (M1). Methoctramine (M2) didn't antagonise the [3H]QNB binding efficiently. Acetylcholine and carbachol increased the intracellular Ca2+ level in 62% and 65% of cultured rat anterior pituitary cells in a dose-dependent manner, and this effect was prevented by pirenzepine. Based on these results we suggest that both M1 and M3 muscarinic receptors are present and active in the majority of cells in the rat anterior pituitary gland, but their physiological role in the adult rat remains to be examined.

Original languageEnglish
Pages (from-to)449-456
Number of pages8
JournalBrain Research Bulletin
Volume48
Issue number4
DOIs
Publication statusPublished - Mar 1 1999

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Muscarinic M3 Receptors
Muscarinic M1 Receptors
Anterior Pituitary Gland
Calcium
Pirenzepine
Muscarinic Receptors
Quinuclidinyl Benzilate
Muscarinic Antagonists
Membranes
Carbachol
Cholinergic Receptors
Atropine
Acetylcholine
4-diphenylacetoxy-1,1-dimethylpiperidinium

Keywords

  • 4-DAMP
  • Acetylcholine
  • Carbachol
  • Fura-2
  • Intracellular calcium
  • Pirenzepine
  • Pituitary
  • QNB

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Muscarinic M1 and M3 receptors are present and increase intracellular calcium in adult rat anterior pituitary gland. / Pintér, Ildikó; Moszkovszkin, Georg; Némethy, Zsolt; Makara, G.

In: Brain Research Bulletin, Vol. 48, No. 4, 01.03.1999, p. 449-456.

Research output: Contribution to journalArticle

Pintér, Ildikó ; Moszkovszkin, Georg ; Némethy, Zsolt ; Makara, G. / Muscarinic M1 and M3 receptors are present and increase intracellular calcium in adult rat anterior pituitary gland. In: Brain Research Bulletin. 1999 ; Vol. 48, No. 4. pp. 449-456.
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