Murine peripherin gene sequences direct Cre recombinase expression to peripheral neurons in transgenic mice

Li Zhou, Virginie Népote, Daniel L. Rowley, Béatrice Levacher, A. Zvara, M. Sántha, Qing Sheng Mi, Michel Simonneau, David M. Donovan

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Spatially and temporally regulated somatic mutations can be achieved by using the Cre/loxP recombination system of bacteriophage P1. To develop a cell type-specific system of gene targeting in the peripheral nervous system, we generated the transgenic mouse lines expressing Cre recombinase under the control of the mouse peripherin gene promoter. The activity of the Cre recombinase during embryonic development was examined by mating the peripherin-Cre transgenic mice to the knock-in Cre-mediated recombination reporter strain, R26R. Analysis of F1 embryos from this cross showed specific excision of loxP-flanked sequences in the dorsal root ganglia, trigeminal ganglia, and olfactory epithelium, in a pattern very similar to the expression of the endogenous mouse peripherin gene, and the previously reported peripherin-lacZ transgenic mice. Thus, the peripherin-Cre mouse described here will provide a valuable tool for Cre-loxP-mediated conditional expression in the peripheral nervous system.

Original languageEnglish
Pages (from-to)68-72
Number of pages5
JournalFEBS Letters
Volume523
Issue number1-3
DOIs
Publication statusPublished - Jul 17 2002

Fingerprint

Peripherins
Transgenic Mice
Neurons
Genes
Peripheral Nervous System
Neurology
Genetic Recombination
Bacteriophage P1
Olfactory Mucosa
Trigeminal Ganglion
Bacteriophages
Gene Targeting
Spinal Ganglia
Embryonic Development
Embryonic Structures
Cre recombinase
Mutation

Keywords

  • Cre
  • Dorsal root ganglion
  • loxP
  • Peripherin
  • ROSA26
  • Sensory neuron

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Murine peripherin gene sequences direct Cre recombinase expression to peripheral neurons in transgenic mice. / Zhou, Li; Népote, Virginie; Rowley, Daniel L.; Levacher, Béatrice; Zvara, A.; Sántha, M.; Mi, Qing Sheng; Simonneau, Michel; Donovan, David M.

In: FEBS Letters, Vol. 523, No. 1-3, 17.07.2002, p. 68-72.

Research output: Contribution to journalArticle

Zhou, Li ; Népote, Virginie ; Rowley, Daniel L. ; Levacher, Béatrice ; Zvara, A. ; Sántha, M. ; Mi, Qing Sheng ; Simonneau, Michel ; Donovan, David M. / Murine peripherin gene sequences direct Cre recombinase expression to peripheral neurons in transgenic mice. In: FEBS Letters. 2002 ; Vol. 523, No. 1-3. pp. 68-72.
@article{23c8ec3e46f44770bc812ef4ac160bfc,
title = "Murine peripherin gene sequences direct Cre recombinase expression to peripheral neurons in transgenic mice",
abstract = "Spatially and temporally regulated somatic mutations can be achieved by using the Cre/loxP recombination system of bacteriophage P1. To develop a cell type-specific system of gene targeting in the peripheral nervous system, we generated the transgenic mouse lines expressing Cre recombinase under the control of the mouse peripherin gene promoter. The activity of the Cre recombinase during embryonic development was examined by mating the peripherin-Cre transgenic mice to the knock-in Cre-mediated recombination reporter strain, R26R. Analysis of F1 embryos from this cross showed specific excision of loxP-flanked sequences in the dorsal root ganglia, trigeminal ganglia, and olfactory epithelium, in a pattern very similar to the expression of the endogenous mouse peripherin gene, and the previously reported peripherin-lacZ transgenic mice. Thus, the peripherin-Cre mouse described here will provide a valuable tool for Cre-loxP-mediated conditional expression in the peripheral nervous system.",
keywords = "Cre, Dorsal root ganglion, loxP, Peripherin, ROSA26, Sensory neuron",
author = "Li Zhou and Virginie N{\'e}pote and Rowley, {Daniel L.} and B{\'e}atrice Levacher and A. Zvara and M. S{\'a}ntha and Mi, {Qing Sheng} and Michel Simonneau and Donovan, {David M.}",
year = "2002",
month = "7",
day = "17",
doi = "10.1016/S0014-5793(02)02936-8",
language = "English",
volume = "523",
pages = "68--72",
journal = "FEBS Letters",
issn = "0014-5793",
publisher = "Elsevier",
number = "1-3",

}

TY - JOUR

T1 - Murine peripherin gene sequences direct Cre recombinase expression to peripheral neurons in transgenic mice

AU - Zhou, Li

AU - Népote, Virginie

AU - Rowley, Daniel L.

AU - Levacher, Béatrice

AU - Zvara, A.

AU - Sántha, M.

AU - Mi, Qing Sheng

AU - Simonneau, Michel

AU - Donovan, David M.

PY - 2002/7/17

Y1 - 2002/7/17

N2 - Spatially and temporally regulated somatic mutations can be achieved by using the Cre/loxP recombination system of bacteriophage P1. To develop a cell type-specific system of gene targeting in the peripheral nervous system, we generated the transgenic mouse lines expressing Cre recombinase under the control of the mouse peripherin gene promoter. The activity of the Cre recombinase during embryonic development was examined by mating the peripherin-Cre transgenic mice to the knock-in Cre-mediated recombination reporter strain, R26R. Analysis of F1 embryos from this cross showed specific excision of loxP-flanked sequences in the dorsal root ganglia, trigeminal ganglia, and olfactory epithelium, in a pattern very similar to the expression of the endogenous mouse peripherin gene, and the previously reported peripherin-lacZ transgenic mice. Thus, the peripherin-Cre mouse described here will provide a valuable tool for Cre-loxP-mediated conditional expression in the peripheral nervous system.

AB - Spatially and temporally regulated somatic mutations can be achieved by using the Cre/loxP recombination system of bacteriophage P1. To develop a cell type-specific system of gene targeting in the peripheral nervous system, we generated the transgenic mouse lines expressing Cre recombinase under the control of the mouse peripherin gene promoter. The activity of the Cre recombinase during embryonic development was examined by mating the peripherin-Cre transgenic mice to the knock-in Cre-mediated recombination reporter strain, R26R. Analysis of F1 embryos from this cross showed specific excision of loxP-flanked sequences in the dorsal root ganglia, trigeminal ganglia, and olfactory epithelium, in a pattern very similar to the expression of the endogenous mouse peripherin gene, and the previously reported peripherin-lacZ transgenic mice. Thus, the peripherin-Cre mouse described here will provide a valuable tool for Cre-loxP-mediated conditional expression in the peripheral nervous system.

KW - Cre

KW - Dorsal root ganglion

KW - loxP

KW - Peripherin

KW - ROSA26

KW - Sensory neuron

UR - http://www.scopus.com/inward/record.url?scp=0037125242&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0037125242&partnerID=8YFLogxK

U2 - 10.1016/S0014-5793(02)02936-8

DO - 10.1016/S0014-5793(02)02936-8

M3 - Article

VL - 523

SP - 68

EP - 72

JO - FEBS Letters

JF - FEBS Letters

SN - 0014-5793

IS - 1-3

ER -