Murine CR1/2 targeted antigenized single-chain antibody fragments induce transient low affinity antibodies and negatively influence an ongoing immune response

J. Prechl, Eszter Molnár, Zsuzsanna Szekeres, Andrea Isaák, Krisztián Papp, P. Balogh, A. Erdei

Research output: Chapter in Book/Report/Conference proceedingConference contribution

5 Citations (Scopus)

Abstract

We have generated a single-chain antibody which recognizes murine CR1/2 and carries a genetically fused influenza hemagglutinin derived peptide. Theoretically such a construct is able to crosslink the B cell antigen receptor and CR1/2 on peptide specific B cells. The construct was able to reach its CR1/2 positive target cells, yet intraperitoneal delivery of the construct elicited an IgM response only slightly exceeding that induced by the free peptide. Providing T cell help by the injection of peptide specific lymphocytes did not alter the response in essence, that is anti-peptide IgG was not detectable even after booster immunizations. When used as a booster vaccine following injection of the peptide in adjuvant, the construct even inhibited the development of IgG1 and IgG3 anti-peptide antibodies. These data indicate that although targeting of antigen to CR1/2 on B cells can enhance transient proliferation or differentiation of antigen specific B cells it cannot induce strong, longlasting humoral immune responses. Furthermore, CR1/2 targeting constructs may negatively influence an ongoing immune reaction.

Original languageEnglish
Title of host publicationAdvances in Experimental Medicine and Biology
PublisherSpringer New York
Pages214-225
Number of pages12
Volume598
ISBN (Print)9780387717654
DOIs
Publication statusPublished - 2007

Publication series

NameAdvances in Experimental Medicine and Biology
Volume598
ISSN (Print)00652598

Fingerprint

Single-Chain Antibodies
Immunoglobulin Fragments
Antibody Affinity
Peptides
Antibodies
Immunoglobulin G
Cells
B-Lymphocyte Differentiation Antigens
B-Lymphocytes
Secondary Immunization
Antigen-antibody reactions
B-Cell Antigen Receptors
Injections
Immunization
Hemagglutinins
Humoral Immunity
T-cells
Lymphocytes
Differentiation Antigens
Human Influenza

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Prechl, J., Molnár, E., Szekeres, Z., Isaák, A., Papp, K., Balogh, P., & Erdei, A. (2007). Murine CR1/2 targeted antigenized single-chain antibody fragments induce transient low affinity antibodies and negatively influence an ongoing immune response. In Advances in Experimental Medicine and Biology (Vol. 598, pp. 214-225). (Advances in Experimental Medicine and Biology; Vol. 598). Springer New York. https://doi.org/10.1007/978-0-387-71767-8_15

Murine CR1/2 targeted antigenized single-chain antibody fragments induce transient low affinity antibodies and negatively influence an ongoing immune response. / Prechl, J.; Molnár, Eszter; Szekeres, Zsuzsanna; Isaák, Andrea; Papp, Krisztián; Balogh, P.; Erdei, A.

Advances in Experimental Medicine and Biology. Vol. 598 Springer New York, 2007. p. 214-225 (Advances in Experimental Medicine and Biology; Vol. 598).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Prechl, J, Molnár, E, Szekeres, Z, Isaák, A, Papp, K, Balogh, P & Erdei, A 2007, Murine CR1/2 targeted antigenized single-chain antibody fragments induce transient low affinity antibodies and negatively influence an ongoing immune response. in Advances in Experimental Medicine and Biology. vol. 598, Advances in Experimental Medicine and Biology, vol. 598, Springer New York, pp. 214-225. https://doi.org/10.1007/978-0-387-71767-8_15
Prechl J, Molnár E, Szekeres Z, Isaák A, Papp K, Balogh P et al. Murine CR1/2 targeted antigenized single-chain antibody fragments induce transient low affinity antibodies and negatively influence an ongoing immune response. In Advances in Experimental Medicine and Biology. Vol. 598. Springer New York. 2007. p. 214-225. (Advances in Experimental Medicine and Biology). https://doi.org/10.1007/978-0-387-71767-8_15
Prechl, J. ; Molnár, Eszter ; Szekeres, Zsuzsanna ; Isaák, Andrea ; Papp, Krisztián ; Balogh, P. ; Erdei, A. / Murine CR1/2 targeted antigenized single-chain antibody fragments induce transient low affinity antibodies and negatively influence an ongoing immune response. Advances in Experimental Medicine and Biology. Vol. 598 Springer New York, 2007. pp. 214-225 (Advances in Experimental Medicine and Biology).
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