Multivalent foldamer-based affinity assay for selective recognition of Aβ oligomers

Gábor Olajos, Éva Bartus, Ildikó Schuster, Gergely Lautner, R. Gyurcsányi, Titanilla Szögi, Lívia Fülöp, Tamás A. Martinek

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Mimicking the molecular recognition functionality of antibodies is a great challenge. Foldamers are attractive candidates because of their relatively small size and designable interaction surface. This paper describes a sandwich type enzyme-linked immunoassay with a tetravalent β-peptide foldamer helix array as capture element and enzyme labeled tracer antibodies. The assay was found to be selective to β-amyloid oligomeric species with surface features transiently present in ongoing aggregation. In optimized conditions, with special emphasis on the foldamer immobilization, a detection limit of 5 pM was achieved with a linear range of 10–500 pM. These results suggest that protein mimetic foldamers can be useful tools in biosensors and affinity assays.

Original languageEnglish
Pages (from-to)131-137
Number of pages7
JournalAnalytica Chimica Acta
Volume960
DOIs
Publication statusPublished - Apr 1 2017

Fingerprint

Oligomers
antibody
Assays
assay
enzyme
Molecular recognition
Antibodies
immunoassay
Biosensing Techniques
Enzymes
Immunoenzyme Techniques
Amyloid
Biosensors
Immobilization
peptide
immobilization
Limit of Detection
Agglomeration
tracer
Peptides

Keywords

  • Antibody mimetics
  • Bioaffinity assay
  • Foldamers
  • Molecular recognition
  • β-Amyloid oligomers

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Environmental Chemistry
  • Spectroscopy

Cite this

Olajos, G., Bartus, É., Schuster, I., Lautner, G., Gyurcsányi, R., Szögi, T., ... Martinek, T. A. (2017). Multivalent foldamer-based affinity assay for selective recognition of Aβ oligomers. Analytica Chimica Acta, 960, 131-137. https://doi.org/10.1016/j.aca.2017.01.013

Multivalent foldamer-based affinity assay for selective recognition of Aβ oligomers. / Olajos, Gábor; Bartus, Éva; Schuster, Ildikó; Lautner, Gergely; Gyurcsányi, R.; Szögi, Titanilla; Fülöp, Lívia; Martinek, Tamás A.

In: Analytica Chimica Acta, Vol. 960, 01.04.2017, p. 131-137.

Research output: Contribution to journalArticle

Olajos, G, Bartus, É, Schuster, I, Lautner, G, Gyurcsányi, R, Szögi, T, Fülöp, L & Martinek, TA 2017, 'Multivalent foldamer-based affinity assay for selective recognition of Aβ oligomers', Analytica Chimica Acta, vol. 960, pp. 131-137. https://doi.org/10.1016/j.aca.2017.01.013
Olajos, Gábor ; Bartus, Éva ; Schuster, Ildikó ; Lautner, Gergely ; Gyurcsányi, R. ; Szögi, Titanilla ; Fülöp, Lívia ; Martinek, Tamás A. / Multivalent foldamer-based affinity assay for selective recognition of Aβ oligomers. In: Analytica Chimica Acta. 2017 ; Vol. 960. pp. 131-137.
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