Multispectral imaging of ion transport in neutral carrier-based cation-selective membranes

Robert E. Gyurcsányi, Ernõ Lindner

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Background: High-resolution spectroscopic imaging of the cross section of ion-selective membranes during real-time electrochemical measurements is termed spectroelectrochemical microscopy (SpECM). SpECM is aimed for optimizing the experimental conditions in mass transport controlled ion-selective electrode (ISE) membranes for improved detection limit. Methods: The SpECM measurements are performed in a thin layer electrochemical cell. The key element of the cell is a membrane strip spacer ring assembly which forms a two compartment electrochemical cell. The cell is placed onto the stage of a microscope and the membrane strip is positioned in the center of the field of view. A slice of the image is focused onto the entrance slit of the imaging spectrometer. Results: SpECM has been used for the determination of the diffusion coefficients of different membrane ingredients and for the quantitative assessment of the charged site concentrations in ISE membranes and membrane plasticizers. In addition, changes in the concentration profiles of the ionophore (free and complexed) and charged mobile sites inside the ISE membranes are documented upon the application of large external voltages. Conclusions: This account demonstrates the power and advantages of SpECM, a multispectral imaging method for investigations of mass transport processes in ISE membranes during electrochemical measurements.

Original languageEnglish
Pages (from-to)792-804
Number of pages13
JournalCytometry Part A
Volume69
Issue number8
DOIs
Publication statusPublished - Aug 1 2006

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Keywords

  • Current/voltage facilitated transport
  • Diffusion coefficients
  • Imaging of ion transport
  • Ion selective membranes
  • Ionic sites
  • Spectroelectrochemical microscopy (SpECM)

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Histology
  • Cell Biology

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