Multipoint interphase FISH in childhood T-acute lymphoblastic leukemia detects subpopulations that carry different chromosome 3 aberrations

Irén Haltrich, Maria Kost-Alimova, Gábor Kovács, Matild Dobos, George Klein, G. Fekete, Stefan Imreh

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

We examined chromosome 3 in 32 childhood acute lymphoblastic leukemia (ALL) bone marrow samples. Using interphase multipoint FISH (mp-FISH), which was developed by our group, with 42 chromosome 3-specific probes, we detected clonal chromosome 3 aberrations in 4 T-cell ALL (T-ALL) cases. Four out of seven T-ALL cases carried 3q trisomies. One T-ALL case carried either trisomy 3 (in 15% of the cells) or a 23-megabase (Mb) 3p13∼p12 deletion in a different subpopulation of cells of 32%. Another T-ALL case had either 3q trisomy in 11% or a 12-Mb 3p12∼p13 deletion in 19% of the cells. The deletions were overlapping. In both cases, the majority of the bone marrow cells (47 and 70%, respectively) were normal chromosome 3 disomics. The interstitial deletions detected harbor a known homozygous deletion region between 72.6 and 78.8 Mb, which has been described in lung and breast tumors and contains the DUTT1/ROBO1 tumor suppressor gene. These deletions detected by mp-FISH would have remained unnoticed by conventional cytogenetics and multiplex FISH, as well as by current methods based on total tumor DNA analysis such as comparative genomic hybridization (CGH), array CGH, and loss of heterozygosity (LOH).

Original languageEnglish
Pages (from-to)54-60
Number of pages7
JournalCancer Genetics and Cytogenetics
Volume172
Issue number1
DOIs
Publication statusPublished - Jan 1 2007

Fingerprint

Precursor T-Cell Lymphoblastic Leukemia-Lymphoma
Chromosomes, Human, Pair 3
Interphase
Precursor Cell Lymphoblastic Leukemia-Lymphoma
Chromosome Aberrations
Comparative Genomic Hybridization
T-Lymphocytes
Loss of Heterozygosity
Trisomy
Tumor Suppressor Genes
Cytogenetics
Bone Marrow Cells
Bone Marrow
Breast Neoplasms
Lung
DNA
Neoplasms
Trisomy 3q Chromosome 3

ASJC Scopus subject areas

  • Cancer Research
  • Genetics
  • Molecular Biology

Cite this

Multipoint interphase FISH in childhood T-acute lymphoblastic leukemia detects subpopulations that carry different chromosome 3 aberrations. / Haltrich, Irén; Kost-Alimova, Maria; Kovács, Gábor; Dobos, Matild; Klein, George; Fekete, G.; Imreh, Stefan.

In: Cancer Genetics and Cytogenetics, Vol. 172, No. 1, 01.01.2007, p. 54-60.

Research output: Contribution to journalArticle

Haltrich, Irén ; Kost-Alimova, Maria ; Kovács, Gábor ; Dobos, Matild ; Klein, George ; Fekete, G. ; Imreh, Stefan. / Multipoint interphase FISH in childhood T-acute lymphoblastic leukemia detects subpopulations that carry different chromosome 3 aberrations. In: Cancer Genetics and Cytogenetics. 2007 ; Vol. 172, No. 1. pp. 54-60.
@article{f1184ef86cde441da14987084609286a,
title = "Multipoint interphase FISH in childhood T-acute lymphoblastic leukemia detects subpopulations that carry different chromosome 3 aberrations",
abstract = "We examined chromosome 3 in 32 childhood acute lymphoblastic leukemia (ALL) bone marrow samples. Using interphase multipoint FISH (mp-FISH), which was developed by our group, with 42 chromosome 3-specific probes, we detected clonal chromosome 3 aberrations in 4 T-cell ALL (T-ALL) cases. Four out of seven T-ALL cases carried 3q trisomies. One T-ALL case carried either trisomy 3 (in 15{\%} of the cells) or a 23-megabase (Mb) 3p13∼p12 deletion in a different subpopulation of cells of 32{\%}. Another T-ALL case had either 3q trisomy in 11{\%} or a 12-Mb 3p12∼p13 deletion in 19{\%} of the cells. The deletions were overlapping. In both cases, the majority of the bone marrow cells (47 and 70{\%}, respectively) were normal chromosome 3 disomics. The interstitial deletions detected harbor a known homozygous deletion region between 72.6 and 78.8 Mb, which has been described in lung and breast tumors and contains the DUTT1/ROBO1 tumor suppressor gene. These deletions detected by mp-FISH would have remained unnoticed by conventional cytogenetics and multiplex FISH, as well as by current methods based on total tumor DNA analysis such as comparative genomic hybridization (CGH), array CGH, and loss of heterozygosity (LOH).",
author = "Ir{\'e}n Haltrich and Maria Kost-Alimova and G{\'a}bor Kov{\'a}cs and Matild Dobos and George Klein and G. Fekete and Stefan Imreh",
year = "2007",
month = "1",
day = "1",
doi = "10.1016/j.cancergencyto.2006.08.004",
language = "English",
volume = "172",
pages = "54--60",
journal = "Cancer Genetics and Cytogenetics",
issn = "0165-4608",
publisher = "Elsevier Inc.",
number = "1",

}

TY - JOUR

T1 - Multipoint interphase FISH in childhood T-acute lymphoblastic leukemia detects subpopulations that carry different chromosome 3 aberrations

AU - Haltrich, Irén

AU - Kost-Alimova, Maria

AU - Kovács, Gábor

AU - Dobos, Matild

AU - Klein, George

AU - Fekete, G.

AU - Imreh, Stefan

PY - 2007/1/1

Y1 - 2007/1/1

N2 - We examined chromosome 3 in 32 childhood acute lymphoblastic leukemia (ALL) bone marrow samples. Using interphase multipoint FISH (mp-FISH), which was developed by our group, with 42 chromosome 3-specific probes, we detected clonal chromosome 3 aberrations in 4 T-cell ALL (T-ALL) cases. Four out of seven T-ALL cases carried 3q trisomies. One T-ALL case carried either trisomy 3 (in 15% of the cells) or a 23-megabase (Mb) 3p13∼p12 deletion in a different subpopulation of cells of 32%. Another T-ALL case had either 3q trisomy in 11% or a 12-Mb 3p12∼p13 deletion in 19% of the cells. The deletions were overlapping. In both cases, the majority of the bone marrow cells (47 and 70%, respectively) were normal chromosome 3 disomics. The interstitial deletions detected harbor a known homozygous deletion region between 72.6 and 78.8 Mb, which has been described in lung and breast tumors and contains the DUTT1/ROBO1 tumor suppressor gene. These deletions detected by mp-FISH would have remained unnoticed by conventional cytogenetics and multiplex FISH, as well as by current methods based on total tumor DNA analysis such as comparative genomic hybridization (CGH), array CGH, and loss of heterozygosity (LOH).

AB - We examined chromosome 3 in 32 childhood acute lymphoblastic leukemia (ALL) bone marrow samples. Using interphase multipoint FISH (mp-FISH), which was developed by our group, with 42 chromosome 3-specific probes, we detected clonal chromosome 3 aberrations in 4 T-cell ALL (T-ALL) cases. Four out of seven T-ALL cases carried 3q trisomies. One T-ALL case carried either trisomy 3 (in 15% of the cells) or a 23-megabase (Mb) 3p13∼p12 deletion in a different subpopulation of cells of 32%. Another T-ALL case had either 3q trisomy in 11% or a 12-Mb 3p12∼p13 deletion in 19% of the cells. The deletions were overlapping. In both cases, the majority of the bone marrow cells (47 and 70%, respectively) were normal chromosome 3 disomics. The interstitial deletions detected harbor a known homozygous deletion region between 72.6 and 78.8 Mb, which has been described in lung and breast tumors and contains the DUTT1/ROBO1 tumor suppressor gene. These deletions detected by mp-FISH would have remained unnoticed by conventional cytogenetics and multiplex FISH, as well as by current methods based on total tumor DNA analysis such as comparative genomic hybridization (CGH), array CGH, and loss of heterozygosity (LOH).

UR - http://www.scopus.com/inward/record.url?scp=33845414453&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33845414453&partnerID=8YFLogxK

U2 - 10.1016/j.cancergencyto.2006.08.004

DO - 10.1016/j.cancergencyto.2006.08.004

M3 - Article

VL - 172

SP - 54

EP - 60

JO - Cancer Genetics and Cytogenetics

JF - Cancer Genetics and Cytogenetics

SN - 0165-4608

IS - 1

ER -