Multiplex AMPLICOR PCR screening for Chlamydia trachomatis and Neisseria gonorrhoeae in women attending non-sexually transmitted disease clinics

M. Bassiri, P. A. Mårdh, M. Domeika, N. Borendal, C. Coll Capdevila, G. Creatsas, S. Douvier, S. Guashino, J. Henry-Suchet, J. Hopwood, M. Kliment, G. Lazdane, H. Mallinson, E. Nagy, A. Neuer, P. Nottin, S. Palacios, G. Penngy, J. Peterek, D. Rebelo & 4 others M. Van Santen, K. Tchoudomirova, A. Templeton, V. Unzeitig

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Abstract

A new PCR kit (AMPLICOR CT/NG; Roche Diagnostic Systems, Inc., Branchburg, N.J.) was used as a screening tool for the detection of Chlamydia trachomatis and Neisserin gonorrhoeae in first-void urine (FVU) specimens from 3,340 asymptomatic women attending European health care units for contraceptive advice or pregnancy termination. All samples were kept frozen (-20°C) prior to testing. Chlamydia-positive samples were retested once by the plasmid-based PCR kit and also by a major outer membrane protein (MOMP) primer-based PCR. Discrepancies were resolved by using the direct immunofluorescence test (DIF) with the centrifuged sediment of the FVU specimens. Samples positive for N. gonorrhoeae were retested bY chromosomal primer-based PCR and verified by a 16S RNA PCR. Of the samples tested, 1.8% were considered inhibitory by using the internal amplification control. Of 81 samples positive for C. trachomatis, 74 samples were positive by both plasmid- and MOMP-based PCRs, 6 samples were positive by plasmid-based PCR and DIF, and one sample was positive by both MOMP-based PCR and DIF. Nine samples (0.3%) were positive for N. gonorrhoeae by the chromosomal primer- based PCR; however, none of the results could be confirmed. The test offers the unique ability to identify inhibition of amplification with the optional internal control.

Original languageEnglish
Pages (from-to)2556-2560
Number of pages5
JournalJournal of Clinical Microbiology
Volume35
Issue number10
Publication statusPublished - Oct 1 1997

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Neisseria gonorrhoeae
Chlamydia trachomatis
Multiplex Polymerase Chain Reaction
Polymerase Chain Reaction
Direct Fluorescent Antibody Technique
Membrane Proteins
Plasmids
Urine
Chlamydia
Gonorrhea
Contraceptive Agents
RNA
Delivery of Health Care
Pregnancy

ASJC Scopus subject areas

  • Microbiology (medical)

Cite this

Bassiri, M., Mårdh, P. A., Domeika, M., Borendal, N., Capdevila, C. C., Creatsas, G., ... Unzeitig, V. (1997). Multiplex AMPLICOR PCR screening for Chlamydia trachomatis and Neisseria gonorrhoeae in women attending non-sexually transmitted disease clinics. Journal of Clinical Microbiology, 35(10), 2556-2560.

Multiplex AMPLICOR PCR screening for Chlamydia trachomatis and Neisseria gonorrhoeae in women attending non-sexually transmitted disease clinics. / Bassiri, M.; Mårdh, P. A.; Domeika, M.; Borendal, N.; Capdevila, C. Coll; Creatsas, G.; Douvier, S.; Guashino, S.; Henry-Suchet, J.; Hopwood, J.; Kliment, M.; Lazdane, G.; Mallinson, H.; Nagy, E.; Neuer, A.; Nottin, P.; Palacios, S.; Penngy, G.; Peterek, J.; Rebelo, D.; Van Santen, M.; Tchoudomirova, K.; Templeton, A.; Unzeitig, V.

In: Journal of Clinical Microbiology, Vol. 35, No. 10, 01.10.1997, p. 2556-2560.

Research output: Contribution to journalArticle

Bassiri, M, Mårdh, PA, Domeika, M, Borendal, N, Capdevila, CC, Creatsas, G, Douvier, S, Guashino, S, Henry-Suchet, J, Hopwood, J, Kliment, M, Lazdane, G, Mallinson, H, Nagy, E, Neuer, A, Nottin, P, Palacios, S, Penngy, G, Peterek, J, Rebelo, D, Van Santen, M, Tchoudomirova, K, Templeton, A & Unzeitig, V 1997, 'Multiplex AMPLICOR PCR screening for Chlamydia trachomatis and Neisseria gonorrhoeae in women attending non-sexually transmitted disease clinics', Journal of Clinical Microbiology, vol. 35, no. 10, pp. 2556-2560.
Bassiri, M. ; Mårdh, P. A. ; Domeika, M. ; Borendal, N. ; Capdevila, C. Coll ; Creatsas, G. ; Douvier, S. ; Guashino, S. ; Henry-Suchet, J. ; Hopwood, J. ; Kliment, M. ; Lazdane, G. ; Mallinson, H. ; Nagy, E. ; Neuer, A. ; Nottin, P. ; Palacios, S. ; Penngy, G. ; Peterek, J. ; Rebelo, D. ; Van Santen, M. ; Tchoudomirova, K. ; Templeton, A. ; Unzeitig, V. / Multiplex AMPLICOR PCR screening for Chlamydia trachomatis and Neisseria gonorrhoeae in women attending non-sexually transmitted disease clinics. In: Journal of Clinical Microbiology. 1997 ; Vol. 35, No. 10. pp. 2556-2560.
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AU - Capdevila, C. Coll

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