Multiple subphases of DNA replication in Chinese hamster ovary (CHO-K1) cells

Gaspar Banfalvi, Marina Mikhailova, Lionel A. Poirier, Ming W. Chou

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18 Citations (Scopus)

Abstract

Replicative DNA synthesis has been measured throughout the S phase in synchronized populations of Chinese hamster ovary cells. When exponentially growing, cells in suspension cultures were subjected to counterflow centrifugal elutriation and the resolution power was increased the biphasic replication profile has been resolved and multiple subphases were distinguished. These replication peaks, termed replication checkpoints, are distributed evenly throughout the S phase. The replication checkpoints have been characterized by their average C values corresponding to 2.05, 2.12, 2.2, 2.45, 2.6, 2.8, 2.95, 3.15, 3.3, 3.45, and 3.85.

Original languageEnglish
Pages (from-to)1493-1498
Number of pages6
JournalDNA and Cell Biology
Volume16
Issue number12
DOIs
Publication statusPublished - Jan 1 1997

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ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cell Biology

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