Multiple subphases of DNA repair and poly (ADP-ribose) synthesis in Chinese hamster ovary (CHO-K1) cells

Edit Szepessy, Gabor Nagy, Zsolt Jenei, Z. Serfőző, Ildiko Csuka, Jill James, G. Bánfalvi

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

The two types of DNA synthesis as well as poly(ADP-ribose) biosynthesis were measured simultaneously in synchronized intact populations of CHO cells throughout the duration of S phase. Naturally occurring DNA fragmentation was detected by random primed oligonucleotide synthesis (ROPS assay). Fractions of synchronous cell populations were obtained by counterflow centrifugal elutriation. By gradually increasing the resolution of centrifugal elutriation multiple non-overlapping repair and replication peaks were obtained. The elutriation profile of DNA repair peaks corresponded to the DNA fragmentation pattern measured by ROPS assay. The number and position of poly(ADP-ribose) peaks during S phase resembled those seen in the DNA replication profile. Our results indicate that PAR synthesis is coupled to DNA replication serving the purpose of genomic stability.

Original languageEnglish
Pages (from-to)201-207
Number of pages7
JournalEuropean Journal of Cell Biology
Volume82
Issue number4
DOIs
Publication statusPublished - Apr 1 2003

Fingerprint

Poly Adenosine Diphosphate Ribose
CHO Cells
DNA Fragmentation
Cricetulus
DNA Replication
S Phase
DNA Repair
Ovary
Genomic Instability
Oligonucleotides
Population
DNA

Keywords

  • Cell cycle
  • Centrifugal elutriation
  • DNA fragmentation
  • NAD
  • Nucleotide incorporation
  • Permeable cells
  • Repair
  • Replication
  • Synchronization

ASJC Scopus subject areas

  • Anatomy
  • Cell Biology

Cite this

Multiple subphases of DNA repair and poly (ADP-ribose) synthesis in Chinese hamster ovary (CHO-K1) cells. / Szepessy, Edit; Nagy, Gabor; Jenei, Zsolt; Serfőző, Z.; Csuka, Ildiko; James, Jill; Bánfalvi, G.

In: European Journal of Cell Biology, Vol. 82, No. 4, 01.04.2003, p. 201-207.

Research output: Contribution to journalArticle

Szepessy, Edit ; Nagy, Gabor ; Jenei, Zsolt ; Serfőző, Z. ; Csuka, Ildiko ; James, Jill ; Bánfalvi, G. / Multiple subphases of DNA repair and poly (ADP-ribose) synthesis in Chinese hamster ovary (CHO-K1) cells. In: European Journal of Cell Biology. 2003 ; Vol. 82, No. 4. pp. 201-207.
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