Multiple roles for Vitamin B6 in plant acclimation to UV-B

Gyula Czégény, L. Kõrösi, Åke Strid, É. Hideg

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Abstract

Direct and indirect roles of vitamin B6 in leaf acclimation to supplementary UV-B radiation are shown in vitamin B6 deficient Arabidopsis thaliana mutant rsr4-1 and C24 wild type. Responses to 4 days of 3.9 kJ m−2 d−1 biologically effective UV-B dose were compared in terms of leaf photochemistry, vitamer content, and antioxidant enzyme activities; complemented with a comprehensive study of vitamer ROS scavenging capacities. Under UV-B, rsr4-1 leaves lost more (34%) photochemical yield than C24 plants (24%). In the absence of UV-B, rsr4-1 leaves contained markedly less pyridoxal-5′-phosphate (PLP) than C24 ones, but levels increased up to the C24 contents in response to UV-B. Activities of class-III ascorbate and glutathione peroxidases increased in C24 leaves upon the UV-B treatment but not in the rsr4-1 mutant. SOD activities remained the same in C24 but decreased by more than 50% in rsr4-1 under UV-B. Although PLP was shown to be an excellent antioxidant in vitro, our results suggest that the UV-B protective role of B6 vitamers is realized indirectly, via supporting peroxidase defence rather than by direct ROS scavenging. We hypothesize that the two defence pathways are linked through the PLP-dependent biosynthesis of cystein and heme, affecting peroxidases.

Original languageEnglish
Article number1259
JournalScientific reports
Volume9
Issue number1
DOIs
Publication statusPublished - Dec 1 2019

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Vitamin B 6
Pyridoxal Phosphate
Acclimatization
Antioxidants
Ascorbate Peroxidases
Photochemistry
Peroxidases
Glutathione Peroxidase
Heme
Arabidopsis
Peroxidase
Radiation
Enzymes

ASJC Scopus subject areas

  • General

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Multiple roles for Vitamin B6 in plant acclimation to UV-B. / Czégény, Gyula; Kõrösi, L.; Strid, Åke; Hideg, É.

In: Scientific reports, Vol. 9, No. 1, 1259, 01.12.2019.

Research output: Contribution to journalArticle

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