Multiple inclusion complex formation of protonated ellipticine with cucurbit[8]uril

thermodynamics and fluorescence properties

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

The encapsulation of protonated ellipticine (EH+) in the cavity of cucurbit[8]uril (CB8) was studied in water at pH 4 with spectrophotometric, fluorescence spectroscopic and isothermal calorimetric measurements. The formation of three types of inclusion complexes was observed depending on the host and guest concentrations. Not only one, but also two EH+ was capable of encapsulation in CB8 in 37 μM EH+ solution and the thermodynamics of the binding steps were revealed. The produced very stable complexes showed markedly different absorption and fluorescence properties. When large excess of CB8 was employed in dilute (0.49 μM) EH+ solution, sequential binding of two CB8 occurred to the monomer alkaloid bringing about a substantial alteration in the fluorescence decay kinetics. The driving force of the 1:2 guest:host complex formation was much lower than that of 1:1 encapsulation.

Original languageEnglish
Pages (from-to)1-7
Number of pages7
JournalSupramolecular Chemistry
DOIs
Publication statusAccepted/In press - Apr 17 2016

Fingerprint

ellipticine
Encapsulation
Fluorescence
Thermodynamics
Alkaloids
Monomers
Kinetics
Water
cucurbit(8)uril

Keywords

  • Alkaloids
  • binding affinity
  • calorimetry
  • fluorescence decay
  • host–guest complex

ASJC Scopus subject areas

  • Chemistry(all)

Cite this

@article{d22cd6b0c589499daef8543f5112e1a7,
title = "Multiple inclusion complex formation of protonated ellipticine with cucurbit[8]uril: thermodynamics and fluorescence properties",
abstract = "The encapsulation of protonated ellipticine (EH+) in the cavity of cucurbit[8]uril (CB8) was studied in water at pH 4 with spectrophotometric, fluorescence spectroscopic and isothermal calorimetric measurements. The formation of three types of inclusion complexes was observed depending on the host and guest concentrations. Not only one, but also two EH+ was capable of encapsulation in CB8 in 37 μM EH+ solution and the thermodynamics of the binding steps were revealed. The produced very stable complexes showed markedly different absorption and fluorescence properties. When large excess of CB8 was employed in dilute (0.49 μM) EH+ solution, sequential binding of two CB8 occurred to the monomer alkaloid bringing about a substantial alteration in the fluorescence decay kinetics. The driving force of the 1:2 guest:host complex formation was much lower than that of 1:1 encapsulation.",
keywords = "Alkaloids, binding affinity, calorimetry, fluorescence decay, host–guest complex",
author = "Z. Miskolczy and L. Bicz{\'o}k and I. Jablonkai",
year = "2016",
month = "4",
day = "17",
doi = "10.1080/10610278.2016.1174237",
language = "English",
pages = "1--7",
journal = "Supramolecular Chemistry",
issn = "1061-0278",
publisher = "Taylor and Francis Ltd.",

}

TY - JOUR

T1 - Multiple inclusion complex formation of protonated ellipticine with cucurbit[8]uril

T2 - thermodynamics and fluorescence properties

AU - Miskolczy, Z.

AU - Biczók, L.

AU - Jablonkai, I.

PY - 2016/4/17

Y1 - 2016/4/17

N2 - The encapsulation of protonated ellipticine (EH+) in the cavity of cucurbit[8]uril (CB8) was studied in water at pH 4 with spectrophotometric, fluorescence spectroscopic and isothermal calorimetric measurements. The formation of three types of inclusion complexes was observed depending on the host and guest concentrations. Not only one, but also two EH+ was capable of encapsulation in CB8 in 37 μM EH+ solution and the thermodynamics of the binding steps were revealed. The produced very stable complexes showed markedly different absorption and fluorescence properties. When large excess of CB8 was employed in dilute (0.49 μM) EH+ solution, sequential binding of two CB8 occurred to the monomer alkaloid bringing about a substantial alteration in the fluorescence decay kinetics. The driving force of the 1:2 guest:host complex formation was much lower than that of 1:1 encapsulation.

AB - The encapsulation of protonated ellipticine (EH+) in the cavity of cucurbit[8]uril (CB8) was studied in water at pH 4 with spectrophotometric, fluorescence spectroscopic and isothermal calorimetric measurements. The formation of three types of inclusion complexes was observed depending on the host and guest concentrations. Not only one, but also two EH+ was capable of encapsulation in CB8 in 37 μM EH+ solution and the thermodynamics of the binding steps were revealed. The produced very stable complexes showed markedly different absorption and fluorescence properties. When large excess of CB8 was employed in dilute (0.49 μM) EH+ solution, sequential binding of two CB8 occurred to the monomer alkaloid bringing about a substantial alteration in the fluorescence decay kinetics. The driving force of the 1:2 guest:host complex formation was much lower than that of 1:1 encapsulation.

KW - Alkaloids

KW - binding affinity

KW - calorimetry

KW - fluorescence decay

KW - host–guest complex

UR - http://www.scopus.com/inward/record.url?scp=84964466634&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84964466634&partnerID=8YFLogxK

U2 - 10.1080/10610278.2016.1174237

DO - 10.1080/10610278.2016.1174237

M3 - Article

SP - 1

EP - 7

JO - Supramolecular Chemistry

JF - Supramolecular Chemistry

SN - 1061-0278

ER -