Multiple elements controlling the expression of wheat high molecular weight glutenin paralogs

Szabolcs Makai, Csaba Éva, L. Tamás, Angéla Juhász

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Analysis of gene expression data generated by high-throughput microarray transcript profiling experiments coupled with cis-regulatory elements enrichment study and cluster analysis can be used to define modular gene programs and regulatory networks. Unfortunately, the high molecular weight glutenin subunits of wheat (Triticum aestivum) are more similar than microarray data alone would allow to distinguish between the three homoeologous gene pairs. However, combining complementary DNA (cDNA) expression libraries with microarray data, a co-expressional network was built that highlighted the hidden differences between these highly similar genes. Duplex clusters of cis-regulatory elements were used to focus the co-expressional network of transcription factors to the putative regulatory network of Glu-1 genes. The focused network helped to identify several transcriptional gene programs in the endosperm. Many of these programs demonstrated a conserved temporal pattern across the studied genotypes; however, few others showed variance. Based on this network, transient gene expression assays were performed with mutated promoters to inspect the control of tissue specificity. Results indicated that the interactions of the ABRE│CBF cluster with distal promoter regions may have a dual role in regulation by both recruiting the transcription complex as well as suppressing it in non-endosperm tissue. A putative model of regulation is discussed.

Original languageEnglish
Pages (from-to)661-672
Number of pages12
JournalFunctional and Integrative Genomics
Volume15
Issue number6
DOIs
Publication statusPublished - Apr 17 2015

Fingerprint

Triticum
Molecular Weight
Genes
Gene Expression
Endosperm
Organ Specificity
Gene Regulatory Networks
Gene Library
Genetic Promoter Regions
Cluster Analysis
Transcription Factors
Complementary DNA
Genotype
glutenin

Keywords

  • cis-regulatory module
  • Co-expression network
  • Endosperm
  • Gene program
  • High molecular weight glutenin
  • Promoter
  • Regulation
  • Tissue specificity
  • Transient gene assay
  • Triticum aestivum

ASJC Scopus subject areas

  • Genetics

Cite this

Multiple elements controlling the expression of wheat high molecular weight glutenin paralogs. / Makai, Szabolcs; Éva, Csaba; Tamás, L.; Juhász, Angéla.

In: Functional and Integrative Genomics, Vol. 15, No. 6, 17.04.2015, p. 661-672.

Research output: Contribution to journalArticle

Makai, Szabolcs ; Éva, Csaba ; Tamás, L. ; Juhász, Angéla. / Multiple elements controlling the expression of wheat high molecular weight glutenin paralogs. In: Functional and Integrative Genomics. 2015 ; Vol. 15, No. 6. pp. 661-672.
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