Multiple divergent picobirnaviruses with functional prokaryotic Shine-Dalgarno ribosome binding sites present in cloacal sample of a diarrheic chicken

Ákos Boros, B. Polgár, P. Pankovics, Hajnalka Fenyvesi, P. Engelmann, Tung Gia Phan, Eric Delwart, G. Reuter

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Picobirnaviruses (PBVs) of family Picobirnaviridae have bisegmented (S1 and S2 segments), double-stranded RNA genomes. In this study a total of N = 12 complete chicken PBVs (ChPBV) segments (N = 5 of S1 and N = 7 of S2, Acc. Nos.: MH425579-90) were determined using viral metagenomic and RT-PCR techniques from a single cloacal sample of a diarrheic chicken. The identified ChPBV segments are unrelated to each other and distant from all of the currently known PBVs. In silico sequence analyses revealed the presence of conserved prokaryotic Shine-Dalgarno-like (SD-like) sequences upstream of the three presumed open reading frames (ORFs) of the S1 and a single presumed ORF of the S2 segments. According to the results of expression analyses in E. coli using 6xHis-tagged recombinant ChPBV segment 1 construct and Western blot these SD-like sequences are functional in vivo suggesting that S1 of study PBVs can contain three ORFs and supporting the bacteriophage-nature of PBVs.

Original languageEnglish
Pages (from-to)62-72
Number of pages11
JournalVirology
Volume525
DOIs
Publication statusPublished - Dec 1 2018

Fingerprint

Picobirnavirus
Ribosomes
Chickens
Binding Sites
Open Reading Frames
Metagenomics
Double-Stranded RNA
Computer Simulation
Bacteriophages
Sequence Analysis
Western Blotting
Genome
Escherichia coli
Polymerase Chain Reaction

Keywords

  • Bacteriophage
  • Complete
  • Metagenomics
  • Phage
  • Picobirnavirus
  • Segment
  • Shine-Dalgarno

ASJC Scopus subject areas

  • Virology

Cite this

Multiple divergent picobirnaviruses with functional prokaryotic Shine-Dalgarno ribosome binding sites present in cloacal sample of a diarrheic chicken. / Boros, Ákos; Polgár, B.; Pankovics, P.; Fenyvesi, Hajnalka; Engelmann, P.; Phan, Tung Gia; Delwart, Eric; Reuter, G.

In: Virology, Vol. 525, 01.12.2018, p. 62-72.

Research output: Contribution to journalArticle

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