Multimodality imaging using SPECT/CT and MRI and ligand functionalized 99mTc-labeled magnetic microbubbles

Åsa A. Barrefelt, Torkel B. Brismar, G. Egri, Peter Aspelin, Annie Olsson, Letizia Oddo, Silvia Margheritelli, Kenneth Caidahl, Gaio Paradossi, Lars Dähne, Rimma Axelsson, Moustapha Hassan

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Background: In the present study, we used multimodal imaging to investigate biodistribution in rats after intravenous administration of a new 99mTc-labeled delivery system consisting of polymer-shelled microbubbles (MBs) functionalized with diethylenetriaminepentaacetic acid (DTPA), thiolated poly(methacrylic acid) (PMAA), chitosan, 1,4,7-triacyclononane-1,4,7-triacetic acid (NOTA), NOTA-super paramagnetic iron oxide nanoparticles (SPION), or DTPA-SPION. Methods: Examinations utilizing planar dynamic scintigraphy and hybrid imaging were performed using a commercially available single-photon emission computed tomography (SPECT)/computed tomography (CT) system. For SPION containing MBs, the biodistribution pattern of 99mTc-labeled NOTA-SPION and DTPA-SPION MBs was investigated and co-registered using fusion SPECT/CT and magnetic resonance imaging (MRI). Moreover, to evaluate the biodistribution, organs were removed and radioactivity was measured and calculated as percentage of injected dose. Results: SPECT/CT and MRI showed that the distribution of 99mTc-labeled ligand-functionalized MBs varied with the type of ligand as well as with the presence of SPION. The highest uptake was observed in the lungs 1 h post injection of 99mTc-labeled DTPA and chitosan MBs, while a similar distribution to the lungs and the liver was seen after the administration of PMAA MBs. The highest counts of 99mTc-labeled NOTA-SPION and DTPA-SPION MBs were observed in the lungs, liver, and kidneys 1 h post injection. The highest counts were observed in the liver, spleen, and kidneys as confirmed by MRI 24 h post injection. Furthermore, the results obtained from organ measurements were in good agreement with those obtained from SPECT/CT. Conclusions: In conclusion, microbubbles functionalized by different ligands can be labeled with radiotracers and utilized for SPECT/CT imaging, while the incorporation of SPION in MB shells enables imaging using MR. Our investigation revealed that biodistribution may be modified using different ligands. Furthermore, using a single contrast agent with fusion SPECT/CT/MR multimodal imaging enables visualization of functional and anatomical information in one image, thus improving the diagnostic benefit for patients.

Original languageEnglish
Pages (from-to)1-14
Number of pages14
JournalEJNMMI Research
Volume3
Issue number1
DOIs
Publication statusPublished - 2013

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Microbubbles
Nanoparticles
Magnetic Resonance Imaging
Ligands
Multimodal Imaging
Acids
Chitosan
Lung
Injections
Liver
Kidney
ferric oxide
Single Photon Emission Computed Tomography Computed Tomography
Pentetic Acid
Radionuclide Imaging
Intravenous Administration
Radioactivity
Contrast Media
Polymers
Spleen

Keywords

  • Tc
  • Biodistribution
  • Microbubbles
  • MRI
  • Multimodality imaging
  • SPECT/CT
  • SPION

ASJC Scopus subject areas

  • Radiology Nuclear Medicine and imaging

Cite this

Multimodality imaging using SPECT/CT and MRI and ligand functionalized 99mTc-labeled magnetic microbubbles. / Barrefelt, Åsa A.; Brismar, Torkel B.; Egri, G.; Aspelin, Peter; Olsson, Annie; Oddo, Letizia; Margheritelli, Silvia; Caidahl, Kenneth; Paradossi, Gaio; Dähne, Lars; Axelsson, Rimma; Hassan, Moustapha.

In: EJNMMI Research, Vol. 3, No. 1, 2013, p. 1-14.

Research output: Contribution to journalArticle

Barrefelt, ÅA, Brismar, TB, Egri, G, Aspelin, P, Olsson, A, Oddo, L, Margheritelli, S, Caidahl, K, Paradossi, G, Dähne, L, Axelsson, R & Hassan, M 2013, 'Multimodality imaging using SPECT/CT and MRI and ligand functionalized 99mTc-labeled magnetic microbubbles', EJNMMI Research, vol. 3, no. 1, pp. 1-14. https://doi.org/10.1186/2191-219X-3-12
Barrefelt, Åsa A. ; Brismar, Torkel B. ; Egri, G. ; Aspelin, Peter ; Olsson, Annie ; Oddo, Letizia ; Margheritelli, Silvia ; Caidahl, Kenneth ; Paradossi, Gaio ; Dähne, Lars ; Axelsson, Rimma ; Hassan, Moustapha. / Multimodality imaging using SPECT/CT and MRI and ligand functionalized 99mTc-labeled magnetic microbubbles. In: EJNMMI Research. 2013 ; Vol. 3, No. 1. pp. 1-14.
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T1 - Multimodality imaging using SPECT/CT and MRI and ligand functionalized 99mTc-labeled magnetic microbubbles

AU - Barrefelt, Åsa A.

AU - Brismar, Torkel B.

AU - Egri, G.

AU - Aspelin, Peter

AU - Olsson, Annie

AU - Oddo, Letizia

AU - Margheritelli, Silvia

AU - Caidahl, Kenneth

AU - Paradossi, Gaio

AU - Dähne, Lars

AU - Axelsson, Rimma

AU - Hassan, Moustapha

PY - 2013

Y1 - 2013

N2 - Background: In the present study, we used multimodal imaging to investigate biodistribution in rats after intravenous administration of a new 99mTc-labeled delivery system consisting of polymer-shelled microbubbles (MBs) functionalized with diethylenetriaminepentaacetic acid (DTPA), thiolated poly(methacrylic acid) (PMAA), chitosan, 1,4,7-triacyclononane-1,4,7-triacetic acid (NOTA), NOTA-super paramagnetic iron oxide nanoparticles (SPION), or DTPA-SPION. Methods: Examinations utilizing planar dynamic scintigraphy and hybrid imaging were performed using a commercially available single-photon emission computed tomography (SPECT)/computed tomography (CT) system. For SPION containing MBs, the biodistribution pattern of 99mTc-labeled NOTA-SPION and DTPA-SPION MBs was investigated and co-registered using fusion SPECT/CT and magnetic resonance imaging (MRI). Moreover, to evaluate the biodistribution, organs were removed and radioactivity was measured and calculated as percentage of injected dose. Results: SPECT/CT and MRI showed that the distribution of 99mTc-labeled ligand-functionalized MBs varied with the type of ligand as well as with the presence of SPION. The highest uptake was observed in the lungs 1 h post injection of 99mTc-labeled DTPA and chitosan MBs, while a similar distribution to the lungs and the liver was seen after the administration of PMAA MBs. The highest counts of 99mTc-labeled NOTA-SPION and DTPA-SPION MBs were observed in the lungs, liver, and kidneys 1 h post injection. The highest counts were observed in the liver, spleen, and kidneys as confirmed by MRI 24 h post injection. Furthermore, the results obtained from organ measurements were in good agreement with those obtained from SPECT/CT. Conclusions: In conclusion, microbubbles functionalized by different ligands can be labeled with radiotracers and utilized for SPECT/CT imaging, while the incorporation of SPION in MB shells enables imaging using MR. Our investigation revealed that biodistribution may be modified using different ligands. Furthermore, using a single contrast agent with fusion SPECT/CT/MR multimodal imaging enables visualization of functional and anatomical information in one image, thus improving the diagnostic benefit for patients.

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