Multilocus genotyping of Amylostereum spp. associated with Sirex noctilio and other woodwasps from Europe reveal clonal lineage introduced to the US

Louela A. Castrillo, Ann E. Hajek, Juan A. Pajares, Iben M. Thomsen, G. Csóka, Shawn C. Kenaley, Ryan M. Kepler, Paula Zamora, Sergio Angeli

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Sirex noctilio is a woodwasp of Eurasian origin that was inadvertently introduced to the southern hemisphere in the 1900s and to North America over a decade ago. Its larvae bore in Pinus spp. and can cause significant mortality in pine plantations. S noctilio is associated with a symbiotic white rot fungus, Amylostereum areolatum, which females inject into trees when they oviposit and which is required for survival of developing larvae. We compared the genetic diversity of A. areolatum isolated from S. noctilio and other woodwasps collected from Europe and from northeastern North America to determine the origin of introduction(s) into the United States. Multilocus genotyping of nuclear ribosomal regions and protein coding genes revealed two widespread multilocus genotypes (MLGs) among the European samples, one of which is present in the US. The other two MLGs associated with S. noctilio in the US represented unique haplotypes. These latter two haplotypes were likely from unrepresented source populations, and together with the introduced widespread haplotype reveal multiple A. areolatum MLGs introduced by S. noctilio and indicate possible multiple S. noctilio introductions to North America from Europe. Our results also showed a lack of fidelity between woodwasp hosts and Amylostereum species.

Original languageEnglish
Pages (from-to)595-604
Number of pages10
JournalFungal Biology
Volume119
Issue number7
DOIs
Publication statusPublished - 2015

Fingerprint

Amylostereum
Sirex noctilio
North America
Haplotypes
genotyping
genotype
Genotype
Larva
haplotypes
larva
Pinus
Ribosomal Proteins
Nuclear Proteins
Southern Hemisphere
plantation
Fungi
white-rot fungi
fungus
larvae
mortality

Keywords

  • Fungal symbiont
  • Genetic diversity
  • Invasive pest
  • Siricid woodwasps
  • Somatic incompatibility

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Plant Science
  • Genetics
  • Infectious Diseases

Cite this

Multilocus genotyping of Amylostereum spp. associated with Sirex noctilio and other woodwasps from Europe reveal clonal lineage introduced to the US. / Castrillo, Louela A.; Hajek, Ann E.; Pajares, Juan A.; Thomsen, Iben M.; Csóka, G.; Kenaley, Shawn C.; Kepler, Ryan M.; Zamora, Paula; Angeli, Sergio.

In: Fungal Biology, Vol. 119, No. 7, 2015, p. 595-604.

Research output: Contribution to journalArticle

Castrillo, Louela A. ; Hajek, Ann E. ; Pajares, Juan A. ; Thomsen, Iben M. ; Csóka, G. ; Kenaley, Shawn C. ; Kepler, Ryan M. ; Zamora, Paula ; Angeli, Sergio. / Multilocus genotyping of Amylostereum spp. associated with Sirex noctilio and other woodwasps from Europe reveal clonal lineage introduced to the US. In: Fungal Biology. 2015 ; Vol. 119, No. 7. pp. 595-604.
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