Multifunctional PEG-carboxylate copolymer coated superparamagnetic iron oxide nanoparticles for biomedical application

E. Illés, M. Szekeres, Ildikó Y. Tóth, Ákos Szabó, B. Iván, Rodica Turcu, Ladislau Vékás, I. Zupkó, György Jaics, E. Tombácz

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Biocompatible magnetite nanoparticles (MNPs) were prepared by post-coating the magnetic nanocores with a synthetic polymer designed specifically to shield the particles from non-specific interaction with cells. Poly(ethylene glycol) methyl ether methacrylate (PEGMA) macromonomers and acrylic acid (AA) small molecular monomers were chemically coupled by quasi-living atom transfer radical polymerization (ATRP) to a comb-like copolymer, P(PEGMA-co-AA) designated here as P(PEGMA-AA). The polymer contains pendant carboxylate moieties near the backbone and PEG side chains. It is able to bind spontaneously to MNPs; stabilize the particles electrostatically via the carboxylate moieties and sterically via the PEG moieties; provide high protein repellency via the structured PEG layer; and anchor bioactive proteins via peptide bond formation with the free carboxylate groups. The presence of the P(PEGMA-AA) coating was verified in XPS experiments. The electrosteric (i.e., combined electrostatic and steric) stabilization is efficient down to pH 4 (at 10 mM ionic strength). Static magnetization and AC susceptibility measurements showed that the P(PEGMA-AA)@MNPs are superparamagnetic with a saturation magnetization value of 55 emu/g and that both single core nanoparticles and multicore structures are present in the samples. The multicore components make our product well suited for magnetic hyperthermia applications (SAR values up to 17.44 W/g). In vitro biocompatibility, cell internalization, and magnetic hyperthermia studies demonstrate the excellent theranostic potential of our product.

Original languageEnglish
Pages (from-to)710-720
Number of pages11
JournalJournal of Magnetism and Magnetic Materials
Volume451
DOIs
Publication statusPublished - Apr 1 2018

Fingerprint

Magnetite Nanoparticles
Magnetite nanoparticles
Iron oxides
iron oxides
carboxylates
Polyethylene glycols
copolymers
Copolymers
magnetite
Nanoparticles
nanoparticles
hyperthermia
acrylic acid
Acrylics
Polymers
proteins
Proteins
coatings
Methyl Ethers
Coatings

Keywords

  • Cell internalization
  • Colloidal stability
  • Core-shell nanoparticles
  • PEGylation
  • Superparamagnetic iron oxide nanoparticles (SPIONs)
  • Theranostics

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics

Cite this

Multifunctional PEG-carboxylate copolymer coated superparamagnetic iron oxide nanoparticles for biomedical application. / Illés, E.; Szekeres, M.; Tóth, Ildikó Y.; Szabó, Ákos; Iván, B.; Turcu, Rodica; Vékás, Ladislau; Zupkó, I.; Jaics, György; Tombácz, E.

In: Journal of Magnetism and Magnetic Materials, Vol. 451, 01.04.2018, p. 710-720.

Research output: Contribution to journalArticle

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T1 - Multifunctional PEG-carboxylate copolymer coated superparamagnetic iron oxide nanoparticles for biomedical application

AU - Illés, E.

AU - Szekeres, M.

AU - Tóth, Ildikó Y.

AU - Szabó, Ákos

AU - Iván, B.

AU - Turcu, Rodica

AU - Vékás, Ladislau

AU - Zupkó, I.

AU - Jaics, György

AU - Tombácz, E.

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AB - Biocompatible magnetite nanoparticles (MNPs) were prepared by post-coating the magnetic nanocores with a synthetic polymer designed specifically to shield the particles from non-specific interaction with cells. Poly(ethylene glycol) methyl ether methacrylate (PEGMA) macromonomers and acrylic acid (AA) small molecular monomers were chemically coupled by quasi-living atom transfer radical polymerization (ATRP) to a comb-like copolymer, P(PEGMA-co-AA) designated here as P(PEGMA-AA). The polymer contains pendant carboxylate moieties near the backbone and PEG side chains. It is able to bind spontaneously to MNPs; stabilize the particles electrostatically via the carboxylate moieties and sterically via the PEG moieties; provide high protein repellency via the structured PEG layer; and anchor bioactive proteins via peptide bond formation with the free carboxylate groups. The presence of the P(PEGMA-AA) coating was verified in XPS experiments. The electrosteric (i.e., combined electrostatic and steric) stabilization is efficient down to pH 4 (at 10 mM ionic strength). Static magnetization and AC susceptibility measurements showed that the P(PEGMA-AA)@MNPs are superparamagnetic with a saturation magnetization value of 55 emu/g and that both single core nanoparticles and multicore structures are present in the samples. The multicore components make our product well suited for magnetic hyperthermia applications (SAR values up to 17.44 W/g). In vitro biocompatibility, cell internalization, and magnetic hyperthermia studies demonstrate the excellent theranostic potential of our product.

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KW - Colloidal stability

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KW - PEGylation

KW - Superparamagnetic iron oxide nanoparticles (SPIONs)

KW - Theranostics

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