Multielectrode culture chamber

a device for long-term recording of bioelectric activities in vitro.

V. Jánossy, A. Tóth, L. Bodocs, P. Imrik, E. Madarász, A. Gyévai

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

A multi-microelectrode culture chamber system was constructed for monitoring simultaneously morphological and electrophysiological development of neural cells in vitro. The setup consisted of a pattern of gold conductor lines evaporated onto a glass substrate and insulated with polyamide. The width of each electrode was 10 microns, and the distance between the electrodes was 60 microns. The electrode patterns were constructed and the uncovering of the electrode tips were carried out by photo-etching. This system allowed us to record spontaneous activities in both explant- and primary monolayer cultures of either rat or mouse spinal cords and forebrains, during neuronal regeneration and maturation.

Original languageEnglish
Pages (from-to)309-320
Number of pages12
JournalActa Biologica Hungarica
Volume41
Issue number4
Publication statusPublished - 1990

Fingerprint

electrodes
Electrodes
electrode
Equipment and Supplies
etching
Microelectrodes
Nylons
Prosencephalon
spinal cord
Gold
gold
Glass
Rats
maturation
Regeneration
glass
Etching
Monolayers
Spinal Cord
explants

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)

Cite this

Multielectrode culture chamber : a device for long-term recording of bioelectric activities in vitro. / Jánossy, V.; Tóth, A.; Bodocs, L.; Imrik, P.; Madarász, E.; Gyévai, A.

In: Acta Biologica Hungarica, Vol. 41, No. 4, 1990, p. 309-320.

Research output: Contribution to journalArticle

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