Multidrug resistance as a dominant molecular marker in transformation of wine yeast

Z. Kozovska, A. Maraz, I. Magyar, J. Subik

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Pure wine yeast cultures are increasingly used in winemaking to perform controlled fermentations and produce wine of reproducible quality. For the genetic manipulation of natural wine yeast strains dominant selective markers are obviously useful. Here we demonstrate the successful use of the mutated PDR3 gene as a dominant molecular marker for the selection of transformants of prototrophic wine yeast Saccharomyces cerevisiae. The selected transformants displayed a multidrug resistance phenotype that was resistant to strobilurin derivatives and azoles used to control pathogenic fungi in agriculture and medicine, respectively. Random amplification of DNA sequences and electrophoretic karyotyping of the host and transformed strains after microvinification experiments resulted in the same gel electrophoresis patterns. The chemical and sensory analysis of experimental wines proved that the used transformants preserved all their useful winemaking properties indicating that the pdr3-9 allele does not deteriorate the technological properties of the transformed wine yeast strain.

Original languageEnglish
Pages (from-to)27-35
Number of pages9
JournalJournal of Biotechnology
Volume92
Issue number1
DOIs
Publication statusPublished - Nov 15 2001

Keywords

  • Dominant marker
  • Multidrug resistance
  • PDR3 gene
  • Transformation
  • Wine yeast

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

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