Multidepth screening of living cells using optical waveguides

R. Horváth, Kaspar Cottier, Henrik C. Pedersen, Jeremy J. Ramsden

Research output: Contribution to journalArticle

60 Citations (Scopus)

Abstract

The use of planar optical waveguides as substrata for label-free, non-invasive monitoring of cells growing on them is demonstrated. Different submicrometre depths (measured from and perpendicular to the substratum surface) can be selected for monitoring. The so-called symmetry waveguide configuration with a low refractive index waveguide support (nanoporous silica with refractive index ∼1.2) and a polystyrene waveguiding film with a heat-embossed grating coupler is exploited to obtain practically useful differences between the penetration depths of different waveguide modes. Robust data processing techniques are developed to obtain quantitative information about the cell refractive index profile perpendicular to the substratum from the measured effective refractive indices of the modes. In particular, a method is introduced with which cell refractive index variations above and below a predefined and tunable depth can be separated using two modes. The technique can be extended to more modes to gain even more comprehensive information from predefined submicrometre slices of the cell layer. The introduced methods are also suitable for monitoring the kinetics of changes in cell refractive index profiles.

Original languageEnglish
Pages (from-to)799-804
Number of pages6
JournalBiosensors and Bioelectronics
Volume24
Issue number4
DOIs
Publication statusPublished - Dec 1 2008

Fingerprint

Refractometry
Optical waveguides
Refractive index
Screening
Cells
Waveguides
Monitoring
Planar waveguides
Polystyrenes
Silicon Dioxide
Labels
Hot Temperature
Silica
Kinetics

Keywords

  • Deep probe waveguide
  • Living cells
  • Multiple depths
  • Optical biosensor
  • Reverse symmetry waveguide

ASJC Scopus subject areas

  • Biophysics
  • Biomedical Engineering
  • Biotechnology
  • Electrochemistry

Cite this

Multidepth screening of living cells using optical waveguides. / Horváth, R.; Cottier, Kaspar; Pedersen, Henrik C.; Ramsden, Jeremy J.

In: Biosensors and Bioelectronics, Vol. 24, No. 4, 01.12.2008, p. 799-804.

Research output: Contribution to journalArticle

Horváth, R. ; Cottier, Kaspar ; Pedersen, Henrik C. ; Ramsden, Jeremy J. / Multidepth screening of living cells using optical waveguides. In: Biosensors and Bioelectronics. 2008 ; Vol. 24, No. 4. pp. 799-804.
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