Multicapillary SDS-gel electrophoresis for the analysis of fluorescently labeled mAb preparations: A high throughput quality control process for the production of QuantiPlasma and PlasmaScan mAb libraries

Andrea Székely, Ákos Szekrényes, Márta Kerékgyártó, Attila Balogh, János Kádas, József Lázár, András Guttman, István Kurucz, László Takács

Research output: Contribution to journalArticle

7 Citations (Scopus)


Molecular heterogeneity of mAb preparations is the result of various co- and post-translational modifications and to contaminants related to the production process. Changes in molecular composition results in alterations of functional performance, therefore quality control and validation of therapeutic or diagnostic protein products is essential. A special case is the consistent production of mAb libraries (QuantiPlasma™ and PlasmaScan™) for proteome profiling, quality control of which represents a challenge because of high number of mAbs (>1000). Here, we devise a generally applicable multicapillary SDS-gel electrophoresis process for the analysis of fluorescently labeled mAb preparations for the high throughput quality control of mAbs of the QuantiPlasma™ and PlasmaScan™ libraries.

Original languageEnglish
Pages (from-to)2155-2162
Number of pages8
Issue number15
Publication statusPublished - Aug 2014



  • Capillary electrophoresis
  • Glycoform
  • MAb proteomics
  • Quality control
  • SDS

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Clinical Biochemistry

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