Multi-site N-glycan mapping study 1

Capillary electrophoresis – laser induced fluorescence

Ákos Szekrényes, Sung Ae Suhr Park, Marcia Santos, Clarence Lew, Aled Jones, Ted Haxo, Michael Kimzey, Shiva Pourkaveh, Zoltán Szabó, Zoran Sosic, Peng Feng, Csaba Váradi, François de l'escaille, Jean Bernard Falmagne, Preeti Sejwal, Thomas Niedringhaus, David Michels, Gordon Freckleton, Melissa Hamm, Anastasiya Manuilov & 11 others Melissa Schwartz, Jiann Kae Luo, Jonathan van Dyck, Pui King Leung, Marcell Olajos, Yingmei Gu, Kai Gao, Wenbo Wang, Jo Wegstein, Samnang Tep, A. Guttman

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

An international team that included 20 independent laboratories from biopharmaceutical companies, universities, analytical contract laboratories and national authorities in the United States, Europe and Asia was formed to evaluate the reproducibility of sample preparation and analysis of N-glycans using capillary electrophoresis of 8-aminopyrene- 1,3,6-trisulfonic acid (APTS)-labeled glycans with laser induced fluorescence (CE-LIF) detection (16 sites) and ultra highperformance liquid chromatography (UHPLC, 12 sites; results to be reported in a subsequent publication). All participants used the same lot of chemicals, samples, reagents, and columns/capillaries to run their assays. Migration time, peak area and peak area percent values were determined for all peaks with >0.1% peak area. Our results demonstrated low variability and high reproducibility, both, within any given site as well across all sites, which indicates that a standard N-glycan analysis platform appropriate for general use (clone selection, process development, lot release, etc.) within the industry can be established.

Original languageEnglish
Pages (from-to)56-64
Number of pages9
JournalmAbs
Volume8
Issue number1
DOIs
Publication statusPublished - Jan 1 2016

Fingerprint

Capillary Electrophoresis
Polysaccharides
Lasers
Fluorescence
Contracts
Liquid Chromatography
Publications
Industry
Clone Cells

Keywords

  • Biotherapeutics
  • Capillary electrophoresis
  • Intercompany study
  • N-glycans

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Cite this

Multi-site N-glycan mapping study 1 : Capillary electrophoresis – laser induced fluorescence. / Szekrényes, Ákos; Park, Sung Ae Suhr; Santos, Marcia; Lew, Clarence; Jones, Aled; Haxo, Ted; Kimzey, Michael; Pourkaveh, Shiva; Szabó, Zoltán; Sosic, Zoran; Feng, Peng; Váradi, Csaba; de l'escaille, François; Falmagne, Jean Bernard; Sejwal, Preeti; Niedringhaus, Thomas; Michels, David; Freckleton, Gordon; Hamm, Melissa; Manuilov, Anastasiya; Schwartz, Melissa; Luo, Jiann Kae; van Dyck, Jonathan; Leung, Pui King; Olajos, Marcell; Gu, Yingmei; Gao, Kai; Wang, Wenbo; Wegstein, Jo; Tep, Samnang; Guttman, A.

In: mAbs, Vol. 8, No. 1, 01.01.2016, p. 56-64.

Research output: Contribution to journalArticle

Szekrényes, Á, Park, SAS, Santos, M, Lew, C, Jones, A, Haxo, T, Kimzey, M, Pourkaveh, S, Szabó, Z, Sosic, Z, Feng, P, Váradi, C, de l'escaille, F, Falmagne, JB, Sejwal, P, Niedringhaus, T, Michels, D, Freckleton, G, Hamm, M, Manuilov, A, Schwartz, M, Luo, JK, van Dyck, J, Leung, PK, Olajos, M, Gu, Y, Gao, K, Wang, W, Wegstein, J, Tep, S & Guttman, A 2016, 'Multi-site N-glycan mapping study 1: Capillary electrophoresis – laser induced fluorescence', mAbs, vol. 8, no. 1, pp. 56-64. https://doi.org/10.1080/19420862.2015.1107687
Szekrényes, Ákos ; Park, Sung Ae Suhr ; Santos, Marcia ; Lew, Clarence ; Jones, Aled ; Haxo, Ted ; Kimzey, Michael ; Pourkaveh, Shiva ; Szabó, Zoltán ; Sosic, Zoran ; Feng, Peng ; Váradi, Csaba ; de l'escaille, François ; Falmagne, Jean Bernard ; Sejwal, Preeti ; Niedringhaus, Thomas ; Michels, David ; Freckleton, Gordon ; Hamm, Melissa ; Manuilov, Anastasiya ; Schwartz, Melissa ; Luo, Jiann Kae ; van Dyck, Jonathan ; Leung, Pui King ; Olajos, Marcell ; Gu, Yingmei ; Gao, Kai ; Wang, Wenbo ; Wegstein, Jo ; Tep, Samnang ; Guttman, A. / Multi-site N-glycan mapping study 1 : Capillary electrophoresis – laser induced fluorescence. In: mAbs. 2016 ; Vol. 8, No. 1. pp. 56-64.
@article{63b9775ed5c84467b3d7dc4ec74bba50,
title = "Multi-site N-glycan mapping study 1: Capillary electrophoresis – laser induced fluorescence",
abstract = "An international team that included 20 independent laboratories from biopharmaceutical companies, universities, analytical contract laboratories and national authorities in the United States, Europe and Asia was formed to evaluate the reproducibility of sample preparation and analysis of N-glycans using capillary electrophoresis of 8-aminopyrene- 1,3,6-trisulfonic acid (APTS)-labeled glycans with laser induced fluorescence (CE-LIF) detection (16 sites) and ultra highperformance liquid chromatography (UHPLC, 12 sites; results to be reported in a subsequent publication). All participants used the same lot of chemicals, samples, reagents, and columns/capillaries to run their assays. Migration time, peak area and peak area percent values were determined for all peaks with >0.1{\%} peak area. Our results demonstrated low variability and high reproducibility, both, within any given site as well across all sites, which indicates that a standard N-glycan analysis platform appropriate for general use (clone selection, process development, lot release, etc.) within the industry can be established.",
keywords = "Biotherapeutics, Capillary electrophoresis, Intercompany study, N-glycans",
author = "{\'A}kos Szekr{\'e}nyes and Park, {Sung Ae Suhr} and Marcia Santos and Clarence Lew and Aled Jones and Ted Haxo and Michael Kimzey and Shiva Pourkaveh and Zolt{\'a}n Szab{\'o} and Zoran Sosic and Peng Feng and Csaba V{\'a}radi and {de l'escaille}, Fran{\cc}ois and Falmagne, {Jean Bernard} and Preeti Sejwal and Thomas Niedringhaus and David Michels and Gordon Freckleton and Melissa Hamm and Anastasiya Manuilov and Melissa Schwartz and Luo, {Jiann Kae} and {van Dyck}, Jonathan and Leung, {Pui King} and Marcell Olajos and Yingmei Gu and Kai Gao and Wenbo Wang and Jo Wegstein and Samnang Tep and A. Guttman",
year = "2016",
month = "1",
day = "1",
doi = "10.1080/19420862.2015.1107687",
language = "English",
volume = "8",
pages = "56--64",
journal = "mAbs",
issn = "1942-0870",
publisher = "Landes Bioscience",
number = "1",

}

TY - JOUR

T1 - Multi-site N-glycan mapping study 1

T2 - Capillary electrophoresis – laser induced fluorescence

AU - Szekrényes, Ákos

AU - Park, Sung Ae Suhr

AU - Santos, Marcia

AU - Lew, Clarence

AU - Jones, Aled

AU - Haxo, Ted

AU - Kimzey, Michael

AU - Pourkaveh, Shiva

AU - Szabó, Zoltán

AU - Sosic, Zoran

AU - Feng, Peng

AU - Váradi, Csaba

AU - de l'escaille, François

AU - Falmagne, Jean Bernard

AU - Sejwal, Preeti

AU - Niedringhaus, Thomas

AU - Michels, David

AU - Freckleton, Gordon

AU - Hamm, Melissa

AU - Manuilov, Anastasiya

AU - Schwartz, Melissa

AU - Luo, Jiann Kae

AU - van Dyck, Jonathan

AU - Leung, Pui King

AU - Olajos, Marcell

AU - Gu, Yingmei

AU - Gao, Kai

AU - Wang, Wenbo

AU - Wegstein, Jo

AU - Tep, Samnang

AU - Guttman, A.

PY - 2016/1/1

Y1 - 2016/1/1

N2 - An international team that included 20 independent laboratories from biopharmaceutical companies, universities, analytical contract laboratories and national authorities in the United States, Europe and Asia was formed to evaluate the reproducibility of sample preparation and analysis of N-glycans using capillary electrophoresis of 8-aminopyrene- 1,3,6-trisulfonic acid (APTS)-labeled glycans with laser induced fluorescence (CE-LIF) detection (16 sites) and ultra highperformance liquid chromatography (UHPLC, 12 sites; results to be reported in a subsequent publication). All participants used the same lot of chemicals, samples, reagents, and columns/capillaries to run their assays. Migration time, peak area and peak area percent values were determined for all peaks with >0.1% peak area. Our results demonstrated low variability and high reproducibility, both, within any given site as well across all sites, which indicates that a standard N-glycan analysis platform appropriate for general use (clone selection, process development, lot release, etc.) within the industry can be established.

AB - An international team that included 20 independent laboratories from biopharmaceutical companies, universities, analytical contract laboratories and national authorities in the United States, Europe and Asia was formed to evaluate the reproducibility of sample preparation and analysis of N-glycans using capillary electrophoresis of 8-aminopyrene- 1,3,6-trisulfonic acid (APTS)-labeled glycans with laser induced fluorescence (CE-LIF) detection (16 sites) and ultra highperformance liquid chromatography (UHPLC, 12 sites; results to be reported in a subsequent publication). All participants used the same lot of chemicals, samples, reagents, and columns/capillaries to run their assays. Migration time, peak area and peak area percent values were determined for all peaks with >0.1% peak area. Our results demonstrated low variability and high reproducibility, both, within any given site as well across all sites, which indicates that a standard N-glycan analysis platform appropriate for general use (clone selection, process development, lot release, etc.) within the industry can be established.

KW - Biotherapeutics

KW - Capillary electrophoresis

KW - Intercompany study

KW - N-glycans

UR - http://www.scopus.com/inward/record.url?scp=84964515417&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84964515417&partnerID=8YFLogxK

U2 - 10.1080/19420862.2015.1107687

DO - 10.1080/19420862.2015.1107687

M3 - Article

VL - 8

SP - 56

EP - 64

JO - mAbs

JF - mAbs

SN - 1942-0870

IS - 1

ER -