Mucosal expression of claudins 2, 3 and 4 in proximal and distal part of duodenum in children with coeliac disease

Dorottya Nagy Szakál, Hajnalka Gyorffy, A. Arató, Áron Cseh, Kriszta Molnár, M. Papp, Antal Dezsofi, G. Verès

Research output: Contribution to journalArticle

41 Citations (Scopus)

Abstract

Duodenal biopsy is an important tool to diagnose coeliac disease (CD); however, the most reliable location of biopsy site is still questionable. Claudins (CLDNs), members of a large family of adherent junction proteins, show characteristic expression pattern in inflammatory disorders; nevertheless, CLDN expression in CD is unknown. This is a comparative study to examine the CLDN 2, 3 and 4 expressions in proximal and distal part of duodenum in children with CD and in controls. Thirty-three children with newly diagnosed CD were enrolled. Fourteen healthy children served as controls. Biopsies from proximal and distal part of duodenum were taken for routine histological analysis. Immunohistochemistry were used to detect CD3+ intraepithelial lymphocytes and CLDN 2, 3 and 4 protein expressions. Macroscopic picture, routine histology and Marsh grade depicted no differences between biopsies taken from proximal or distal part of duodenum. However, CLDN 2 expression was significantly increased in severe form of coeliac disease in bulb and in distal duodenum, and in distal part of non-severe coeliac patients, in comparison to controls. Similar association was found concerning CLDN 3 expression. Expression of CLDN 4 was similar in all groups studied. Both proximal and distal mucosal duodenal biopsies are suitable for diagnosing villous atrophy in patients with CD. Increased expressions of CLDN 2 and 3 suggest structural changes of tight junction in coeliac disease which may be, at least in part, responsible for increased permeability and proliferation observed in coeliac disease.

Original languageEnglish
Pages (from-to)245-250
Number of pages6
JournalVirchows Archiv
Volume456
Issue number3
DOIs
Publication statusPublished - Mar 2010

Fingerprint

Claudins
Celiac Disease
Duodenum
Biopsy
Wetlands
Tight Junctions
Abdomen
Atrophy
Permeability
Histology
Proteins
Immunohistochemistry
Lymphocytes

Keywords

  • Biopsy
  • Claudin
  • Coeliac disease
  • Duodenum
  • Immunohistochemistry

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Cell Biology
  • Molecular Biology
  • Medicine(all)

Cite this

Mucosal expression of claudins 2, 3 and 4 in proximal and distal part of duodenum in children with coeliac disease. / Nagy Szakál, Dorottya; Gyorffy, Hajnalka; Arató, A.; Cseh, Áron; Molnár, Kriszta; Papp, M.; Dezsofi, Antal; Verès, G.

In: Virchows Archiv, Vol. 456, No. 3, 03.2010, p. 245-250.

Research output: Contribution to journalArticle

Nagy Szakál, Dorottya ; Gyorffy, Hajnalka ; Arató, A. ; Cseh, Áron ; Molnár, Kriszta ; Papp, M. ; Dezsofi, Antal ; Verès, G. / Mucosal expression of claudins 2, 3 and 4 in proximal and distal part of duodenum in children with coeliac disease. In: Virchows Archiv. 2010 ; Vol. 456, No. 3. pp. 245-250.
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