Mu opioid receptor mRNA expression, binding, and functional coupling to G-proteins in human epileptic hippocampus

Manola Cuellar-Herrera, Ana Luisa Velasco, Francisco Velasco, Laura Chavez, Sandra Orozco-Suarez, Guliz Armagan, Ezgi Turunc, Engin Bojnik, Ayfer Yalcin, Sandor Benyhe, Anna Borsodi, Mario Alonso-Vanegas, Luisa Rocha

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Abstract

Mu opioid receptors (MOR) are known to be involved in seizure activity. The main goal of the present study was to characterize the MOR mRNA expression, binding, as well as G protein activation mediated by these receptors in epileptic hippocampus of patients with pharmacoresistant mesial temporal lobe epilepsy (TLE). In contrast with autopsy samples, hippocampus obtained from patients with mesial TLE demonstrated enhanced MOR mRNA expression (116%). Saturation binding experiments revealed significantly higher (60%) B max values for the mesial TLE group, whereas the K d values were not statistically different. Although mesial TLE group demonstrated high levels of basal binding for the G proteins (136%), DAMGO-stimulated [ 35S]GTPγS binding did not demonstrate significant alterations. In conclusion, our present data provide strong evidence that the epileptic hippocampus of patients with pharmacoresistant mesial TLE presents significant alterations in MOR. Such changes may represent adaptive mechanisms to compensate for other as yet unknown alterations.

Original languageEnglish
Pages (from-to)122-127
Number of pages6
JournalHippocampus
Volume22
Issue number2
DOIs
Publication statusPublished - Feb 1 2012

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Keywords

  • Binding
  • G-protein
  • Hippocampus
  • MRNA
  • Mesial temporal lobe epilepsy
  • Mu opiod receptor

ASJC Scopus subject areas

  • Cognitive Neuroscience

Cite this

Cuellar-Herrera, M., Velasco, A. L., Velasco, F., Chavez, L., Orozco-Suarez, S., Armagan, G., Turunc, E., Bojnik, E., Yalcin, A., Benyhe, S., Borsodi, A., Alonso-Vanegas, M., & Rocha, L. (2012). Mu opioid receptor mRNA expression, binding, and functional coupling to G-proteins in human epileptic hippocampus. Hippocampus, 22(2), 122-127. https://doi.org/10.1002/hipo.20891