mRNA population in the liver, kidney and brain of young and senescent mice

Analysis of in vitro translation products

Kaijiro Anzai, Chiyuki Imazato, S. Goto

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Possible alterations in the population of poly(A)(+)mRNA during ageing were investigated by translation in vitro of poly(A)(+)mRNA from the liver, kidney and brain of male ddY mice of different ages. [35S]Methionine-labeled translation products were analysed by two-dimensional polyacrylamide gel electrophoresis followed by fluorography. A protein product with a molecular weight of 30 000 and isoelectric point of 6.5 was reproducibly observed only in the fluorograms of translation products of poly(a)(+)mRNA derived from the livers of senescent mice (24.5 months old). However, no age-related change was detected in the translation products of the kidney and brain. These results suggest that gene expression in liver cells changes at the level of the population of cytoplasmic poly(A)(+)mRNA during ageing.

Original languageEnglish
Pages (from-to)137-150
Number of pages14
JournalMechanisms of Ageing and Development
Volume23
Issue number2
DOIs
Publication statusPublished - 1983

Fingerprint

Liver
Brain
Kidney
Messenger RNA
Population
Photofluorography
Aging of materials
Electrophoresis, Gel, Two-Dimensional
Isoelectric Point
Electrophoresis
Gene expression
Methionine
Molecular Weight
Molecular weight
Gene Expression
In Vitro Techniques
Proteins

Keywords

  • Ageing
  • In vitro translation
  • Poly(A)(+)mRNA
  • Two-dimensional gel electrophoresis

ASJC Scopus subject areas

  • Ageing
  • Biochemistry
  • Developmental Biology
  • Developmental Neuroscience

Cite this

mRNA population in the liver, kidney and brain of young and senescent mice : Analysis of in vitro translation products. / Anzai, Kaijiro; Imazato, Chiyuki; Goto, S.

In: Mechanisms of Ageing and Development, Vol. 23, No. 2, 1983, p. 137-150.

Research output: Contribution to journalArticle

@article{5b5cd1bdcf02443991414f9b5f6c5d35,
title = "mRNA population in the liver, kidney and brain of young and senescent mice: Analysis of in vitro translation products",
abstract = "Possible alterations in the population of poly(A)(+)mRNA during ageing were investigated by translation in vitro of poly(A)(+)mRNA from the liver, kidney and brain of male ddY mice of different ages. [35S]Methionine-labeled translation products were analysed by two-dimensional polyacrylamide gel electrophoresis followed by fluorography. A protein product with a molecular weight of 30 000 and isoelectric point of 6.5 was reproducibly observed only in the fluorograms of translation products of poly(a)(+)mRNA derived from the livers of senescent mice (24.5 months old). However, no age-related change was detected in the translation products of the kidney and brain. These results suggest that gene expression in liver cells changes at the level of the population of cytoplasmic poly(A)(+)mRNA during ageing.",
keywords = "Ageing, In vitro translation, Poly(A)(+)mRNA, Two-dimensional gel electrophoresis",
author = "Kaijiro Anzai and Chiyuki Imazato and S. Goto",
year = "1983",
doi = "10.1016/0047-6374(83)90063-5",
language = "English",
volume = "23",
pages = "137--150",
journal = "Mechanisms of Ageing and Development",
issn = "0047-6374",
publisher = "Elsevier Ireland Ltd",
number = "2",

}

TY - JOUR

T1 - mRNA population in the liver, kidney and brain of young and senescent mice

T2 - Analysis of in vitro translation products

AU - Anzai, Kaijiro

AU - Imazato, Chiyuki

AU - Goto, S.

PY - 1983

Y1 - 1983

N2 - Possible alterations in the population of poly(A)(+)mRNA during ageing were investigated by translation in vitro of poly(A)(+)mRNA from the liver, kidney and brain of male ddY mice of different ages. [35S]Methionine-labeled translation products were analysed by two-dimensional polyacrylamide gel electrophoresis followed by fluorography. A protein product with a molecular weight of 30 000 and isoelectric point of 6.5 was reproducibly observed only in the fluorograms of translation products of poly(a)(+)mRNA derived from the livers of senescent mice (24.5 months old). However, no age-related change was detected in the translation products of the kidney and brain. These results suggest that gene expression in liver cells changes at the level of the population of cytoplasmic poly(A)(+)mRNA during ageing.

AB - Possible alterations in the population of poly(A)(+)mRNA during ageing were investigated by translation in vitro of poly(A)(+)mRNA from the liver, kidney and brain of male ddY mice of different ages. [35S]Methionine-labeled translation products were analysed by two-dimensional polyacrylamide gel electrophoresis followed by fluorography. A protein product with a molecular weight of 30 000 and isoelectric point of 6.5 was reproducibly observed only in the fluorograms of translation products of poly(a)(+)mRNA derived from the livers of senescent mice (24.5 months old). However, no age-related change was detected in the translation products of the kidney and brain. These results suggest that gene expression in liver cells changes at the level of the population of cytoplasmic poly(A)(+)mRNA during ageing.

KW - Ageing

KW - In vitro translation

KW - Poly(A)(+)mRNA

KW - Two-dimensional gel electrophoresis

UR - http://www.scopus.com/inward/record.url?scp=0021014329&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0021014329&partnerID=8YFLogxK

U2 - 10.1016/0047-6374(83)90063-5

DO - 10.1016/0047-6374(83)90063-5

M3 - Article

VL - 23

SP - 137

EP - 150

JO - Mechanisms of Ageing and Development

JF - Mechanisms of Ageing and Development

SN - 0047-6374

IS - 2

ER -