Mouse euchromatin specific 'genome-painting' with a LINE probe: A rapid method for identification and mapping of human chromosomes in mouse-human microcell hybrids by two-color FISH

R. Katona, Anna Szeles, Gyula Hadlaczky

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

We describe the use of a long interspersed repetitive sequence (mCPE1.51) for mouse euchromatin specific 'genome-painting'. In fluorescence in situ hybridization (FISH) experiments, this probe was suitable for identification of the mouse genome and disclosure of translocations of mouse chromosome segments to chromosomes of different species without suppression hybridization. The euchromatin specificity of the probe allowed the discrimination between euchromatin and heterochromatin of mouse chromosomes. Simultaneous hybridization of the biotinylated mouse specific genome-painting probe and a digoxigenin-labeled human chromosome 3-specific cosmid probe to metaphase spreads of mouse-human microcell hybrid carrying a single deleted human chromosome 3 on a mouse fibrosarcoma background, allowed rapid identification and mapping of human chromosome 3.

Original languageEnglish
Pages (from-to)131-135
Number of pages5
JournalHereditas
Volume124
Issue number2
DOIs
Publication statusPublished - 1996

Fingerprint

Euchromatin
Paintings
Human Chromosomes
Fluorescence In Situ Hybridization
Color
Genome
Chromosomes, Human, Pair 3
Chromosomes
Interspersed Repetitive Sequences
Digoxigenin
Cosmids
Heterochromatin
Fibrosarcoma
Disclosure
Metaphase

ASJC Scopus subject areas

  • Genetics

Cite this

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AU - Szeles, Anna

AU - Hadlaczky, Gyula

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