Morphotype of bacteroids in different legumes correlates with the number and type of symbiotic NCR peptides

Jesús Montiel, J. Allan Downie, Attila Farkas, Péter Bihari, Róbert Herczeg, Balázs Bálint, Peter Mergaert, A. Kereszt, É. Kondorosi

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

In legume nodules, rhizobia differentiate into nitrogen-fixing forms called bacteroids, which are enclosed by a plant membrane in an organelle-like structure called the symbiosome. In the Inverted Repeat-Lacking Clade (IRLC) of legumes, this differentiation is terminal due to irreversible loss of cell division ability and is associated with genome amplification and different morphologies of the bacteroids that can be swollen, elongated, spherical, and elongated-branched, depending on the host plant. In Medicago truncatula, this process is orchestrated by nodule-specific cysteine-rich peptides (NCRs) delivered into developing bacteroids. Here, we identified the predicted NCR proteins in 10 legumes representing different subclades of the IRLC with distinct bacteroid morphotypes. Analysis of their expression and predicted sequences establishes correlations between the composition of the NCR family and the morphotypes of bacteroids. Although NCRs have a single origin, their evolution has followed different routes in individual lineages, and enrichment and diversification of cationic peptides has resulted in the ability to impose major morphological changes on the endosymbionts. The wide range of effects provoked by NCRs such as cell enlargement, membrane alterations and permeabilization, and biofilm and vesicle formation is dependent on the amino acid composition and charge of the peptides. These effects are strongly influenced by the rhizobial surface polysaccharides that affect NCR-induced differentiation and survival of rhizobia in nodule cells.

Original languageEnglish
Pages (from-to)5041-5046
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume114
Issue number19
DOIs
Publication statusPublished - May 9 2017

Fingerprint

Fabaceae
Rhizobium
Peptides
Medicago truncatula
Cell Enlargement
Biofilms
Organelles
Cell Division
Polysaccharides
Cysteine
Nitrogen
Cell Membrane
Genome
Amino Acids
Membranes
Proteins

Keywords

  • Legume
  • Nitrogen-fixing bacteroids
  • Nodule
  • Nodule-specific cysteine-rich peptides
  • Symbiosis

ASJC Scopus subject areas

  • General

Cite this

Morphotype of bacteroids in different legumes correlates with the number and type of symbiotic NCR peptides. / Montiel, Jesús; Downie, J. Allan; Farkas, Attila; Bihari, Péter; Herczeg, Róbert; Bálint, Balázs; Mergaert, Peter; Kereszt, A.; Kondorosi, É.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 114, No. 19, 09.05.2017, p. 5041-5046.

Research output: Contribution to journalArticle

Montiel, Jesús ; Downie, J. Allan ; Farkas, Attila ; Bihari, Péter ; Herczeg, Róbert ; Bálint, Balázs ; Mergaert, Peter ; Kereszt, A. ; Kondorosi, É. / Morphotype of bacteroids in different legumes correlates with the number and type of symbiotic NCR peptides. In: Proceedings of the National Academy of Sciences of the United States of America. 2017 ; Vol. 114, No. 19. pp. 5041-5046.
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