Morphological study of organotypic cerebellar cultures

J. Takács, F. Metzger

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Organotypic cerebellar cultures from 8-days-old (P8) mouse pups were studied following 11 days of in vitro (11DIV) culturing. The cerebellar cytoarchitectonic structure was maintained in most parasagittal cerebellar cortical slice cultures (also containing the deep cerebellar nuclei). The two main extrinsic excitatory inputs (the climbing and the mossy fibers) seem to be replaced by other axonal types: in the molecular layer mostly by parallel fibers (for climbing fibers) and in the granular layer by intrinsic mossy fiber collaterals of local excitatory interneurons, the unipolar brush cells. However, in a few organotypic cultures, which (although preserving the trilaminar cerebellar cortical structure) were "granuloprival" but also contained some of the deep cerebellar nuclei, the participation of extracortical axons from the deep cerebellar nuclei in the replacement of the missing afferents is suggested.

Original languageEnglish
Pages (from-to)187-204
Number of pages18
JournalActa biologica Hungarica
Issue number1-2
Publication statusPublished - May 2 2002



  • Cerebellum
  • Organotypic culture
  • Synaptic plasticity
  • Ultrastructure

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Environmental Science(all)
  • Neurology

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