Morphological examination of the juxtaglomerular apparatus following the fluorescence microscopic demonstration of sympathetic nerves

Z. Nemes, E. Bodolay, K. Pecze, P. Endes

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1 Citation (Scopus)

Abstract

Several catecholamine demonstrating fluorescence techniques based on the formaldehyde condensation principle were examined for the possibility of juxtaglomerular granules. Freezing and thawing cause disruption of the granules. Short formalin fixation does not prevent this damage. However, the granules were stained successfully after freeze drying. The adaptation of Endes's trichrome method on the basis of the classical catecholamine technique of Falck and Owman resulted in a reliable practical method. Procedures introduced into the trichrome methods to increase the color and the contrast intensity of the juxtaglomerular granules and to stain the elastic membranes are described.

Original languageEnglish
Pages (from-to)71-82
Number of pages12
JournalActa Morphologica Academiae Scientiarum Hungaricae
Volume23
Issue number1
Publication statusPublished - 1975

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Juxtaglomerular Apparatus
Fluorescence
Formaldehyde
Catecholamines
Freeze Drying
Freezing
Coloring Agents
Color
Membranes

ASJC Scopus subject areas

  • Anatomy

Cite this

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abstract = "Several catecholamine demonstrating fluorescence techniques based on the formaldehyde condensation principle were examined for the possibility of juxtaglomerular granules. Freezing and thawing cause disruption of the granules. Short formalin fixation does not prevent this damage. However, the granules were stained successfully after freeze drying. The adaptation of Endes's trichrome method on the basis of the classical catecholamine technique of Falck and Owman resulted in a reliable practical method. Procedures introduced into the trichrome methods to increase the color and the contrast intensity of the juxtaglomerular granules and to stain the elastic membranes are described.",
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