Morphological events in cultures of mechanically isolated maize microspores

Bohuš Obert, László Szabó, Judit Mitykó, Anna Preťová, B. Barnabás

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

In its androgenic response, maize is considered to be a recalcitrant plant. We used mechanically isolated microspores of maize genotype A18 to establish a responsive microspore culture of maize. Morphological events occurring during the first days of maize androgenesis in a microspore culture were observed and described, and some morphological markers for distinguishing between embryogenic microspores and nonembryogenic microspores were identified. It was found that the enlargement of microspores during the first days in culture and the 'star-like' organization of the cytoplasm inside the microspore are connected with reprogramming of the developmental pathway in maize microspores. Some differences were also found in the surface wall architecture of embryogenic microspores. Fertile plants were successfully recovered from microspore-originated structures.

Original languageEnglish
Pages (from-to)775-782
Number of pages8
JournalIn Vitro Cellular and Developmental Biology - Plant
Volume41
Issue number6
DOIs
Publication statusPublished - Nov 2005

Fingerprint

microspores
Zea mays
corn
Cytoplasm
Genotype
androgenesis
cytoplasm
genotype

Keywords

  • In vitro androgenesis
  • Microspore
  • Zea mays L.

ASJC Scopus subject areas

  • Plant Science
  • Cell Biology
  • Developmental Biology
  • Embryology

Cite this

Morphological events in cultures of mechanically isolated maize microspores. / Obert, Bohuš; Szabó, László; Mitykó, Judit; Preťová, Anna; Barnabás, B.

In: In Vitro Cellular and Developmental Biology - Plant, Vol. 41, No. 6, 11.2005, p. 775-782.

Research output: Contribution to journalArticle

Obert, Bohuš ; Szabó, László ; Mitykó, Judit ; Preťová, Anna ; Barnabás, B. / Morphological events in cultures of mechanically isolated maize microspores. In: In Vitro Cellular and Developmental Biology - Plant. 2005 ; Vol. 41, No. 6. pp. 775-782.
@article{ed78b3848d834cd68ffb104aa103e749,
title = "Morphological events in cultures of mechanically isolated maize microspores",
abstract = "In its androgenic response, maize is considered to be a recalcitrant plant. We used mechanically isolated microspores of maize genotype A18 to establish a responsive microspore culture of maize. Morphological events occurring during the first days of maize androgenesis in a microspore culture were observed and described, and some morphological markers for distinguishing between embryogenic microspores and nonembryogenic microspores were identified. It was found that the enlargement of microspores during the first days in culture and the 'star-like' organization of the cytoplasm inside the microspore are connected with reprogramming of the developmental pathway in maize microspores. Some differences were also found in the surface wall architecture of embryogenic microspores. Fertile plants were successfully recovered from microspore-originated structures.",
keywords = "In vitro androgenesis, Microspore, Zea mays L.",
author = "Bohuš Obert and L{\'a}szl{\'o} Szab{\'o} and Judit Mityk{\'o} and Anna Preťov{\'a} and B. Barnab{\'a}s",
year = "2005",
month = "11",
doi = "10.1079/IVP2005701",
language = "English",
volume = "41",
pages = "775--782",
journal = "In Vitro Cellular and Developmental Biology - Plant",
issn = "1054-5476",
publisher = "Springer New York",
number = "6",

}

TY - JOUR

T1 - Morphological events in cultures of mechanically isolated maize microspores

AU - Obert, Bohuš

AU - Szabó, László

AU - Mitykó, Judit

AU - Preťová, Anna

AU - Barnabás, B.

PY - 2005/11

Y1 - 2005/11

N2 - In its androgenic response, maize is considered to be a recalcitrant plant. We used mechanically isolated microspores of maize genotype A18 to establish a responsive microspore culture of maize. Morphological events occurring during the first days of maize androgenesis in a microspore culture were observed and described, and some morphological markers for distinguishing between embryogenic microspores and nonembryogenic microspores were identified. It was found that the enlargement of microspores during the first days in culture and the 'star-like' organization of the cytoplasm inside the microspore are connected with reprogramming of the developmental pathway in maize microspores. Some differences were also found in the surface wall architecture of embryogenic microspores. Fertile plants were successfully recovered from microspore-originated structures.

AB - In its androgenic response, maize is considered to be a recalcitrant plant. We used mechanically isolated microspores of maize genotype A18 to establish a responsive microspore culture of maize. Morphological events occurring during the first days of maize androgenesis in a microspore culture were observed and described, and some morphological markers for distinguishing between embryogenic microspores and nonembryogenic microspores were identified. It was found that the enlargement of microspores during the first days in culture and the 'star-like' organization of the cytoplasm inside the microspore are connected with reprogramming of the developmental pathway in maize microspores. Some differences were also found in the surface wall architecture of embryogenic microspores. Fertile plants were successfully recovered from microspore-originated structures.

KW - In vitro androgenesis

KW - Microspore

KW - Zea mays L.

UR - http://www.scopus.com/inward/record.url?scp=32944464284&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=32944464284&partnerID=8YFLogxK

U2 - 10.1079/IVP2005701

DO - 10.1079/IVP2005701

M3 - Article

AN - SCOPUS:32944464284

VL - 41

SP - 775

EP - 782

JO - In Vitro Cellular and Developmental Biology - Plant

JF - In Vitro Cellular and Developmental Biology - Plant

SN - 1054-5476

IS - 6

ER -