Morphological evaluation of experimental autologous rectus fascia sheath vascular grafts used for arterial replacement in a dog model

Péter Csébi, Csaba Jakab, Attila Patonai, Attila Arany-Tóth, L. Kóbori, Tibor Németh

Research output: Contribution to journalArticle

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Abstract

Although experimental autologous patch or tubular conduit vascular grafts made from the internal rectus fascia sheath (IRFS) have been reported in the literature, thorough morphological evaluation and verification of the histological arterialisation of such grafts are lacking. Four purpose-bred Beagle dogs were utilised to create eight arterial internal rectus fascia sheath (ARFS) grafts implanted between bisected ends of the external iliac arteries. Four out of the eight ARFS grafts were patent after three months. Haematoxylin-eosin and Azan staining verified that the grafts gained a vessel-like layered structure with the presence of large amounts of collagen fibres. Although the inner surface of the intact IRFS was originally covered with claudin-5-negative and pancytokeratin-positive mesothelial cells in control samples, the internal cells of the ARFS grafts became claudin- 5 positive and pancytokeratin negative like in intact arteries. Spindle-shaped cells of the wall of ARFS grafts were α-smooth muscle actin (α-SMA) positive just like the smooth muscle cells of intact arteries, but α-SMA immunoreactivity was negative in the intact IRFS. According to these findings, the fibroblast cells of the ARFS graft have changed into myofibroblast cells. The study has proved that ARFS grafts may be used as an alternative in arterial replacement, since the graft becomes morphologically and functionally similar to the host vessel via arterialisation.

Original languageEnglish
Pages (from-to)429-438
Number of pages10
JournalActa Veterinaria Hungarica
Volume62
Issue number4
DOIs
Publication statusPublished - 2014

Fingerprint

fascia
Fascia
blood vessels
Blood Vessels
Dogs
Transplants
dogs
Claudin-5
smooth muscle
arteries
iliac artery
Arteries
patents
Myofibroblasts
cells
Iliac Artery
Beagle
Hematoxylin
Eosine Yellowish-(YS)
myocytes

Keywords

  • Alpha-SMA
  • Autologous vascular graft
  • Claudin-5
  • Desmin
  • Immunohistochemistry
  • Pancytokeratin
  • Rectus fascia sheath
  • Veterinary field

ASJC Scopus subject areas

  • veterinary(all)

Cite this

Morphological evaluation of experimental autologous rectus fascia sheath vascular grafts used for arterial replacement in a dog model. / Csébi, Péter; Jakab, Csaba; Patonai, Attila; Arany-Tóth, Attila; Kóbori, L.; Németh, Tibor.

In: Acta Veterinaria Hungarica, Vol. 62, No. 4, 2014, p. 429-438.

Research output: Contribution to journalArticle

Csébi, Péter ; Jakab, Csaba ; Patonai, Attila ; Arany-Tóth, Attila ; Kóbori, L. ; Németh, Tibor. / Morphological evaluation of experimental autologous rectus fascia sheath vascular grafts used for arterial replacement in a dog model. In: Acta Veterinaria Hungarica. 2014 ; Vol. 62, No. 4. pp. 429-438.
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